Engineering New REU Approaches to Challenges in MultiTERM

REU 设计新方法应对 MultiTERM 挑战

• 批准号: 1659663
• 负责人: Anthony Atala
• 金额: $ 36.64万
• 依托单位: Wake Forest University School of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1659663&HistoricalAwards=false
• 关键词: Engineering; New; REU; Approaches; Challenges

This three year Research Experiences for Undergraduates (REU) Site program, Engineering New REU Approaches to Challenges in MultiTERM at the Wake Forest Institute for Regenerative Medicine (WFIRM), will provide research opportunities at the interface of engineering and biology in challenging areas of tissue engineering and regenerative medicine (TERM) to a diverse cohort of undergraduate students from underrepresented groups with limited access to research facilities. The proposed research projects span multiple physiological systems (cardiovascular, musculoskeletal, urological, and endocrine), providing REU students a unique opportunity to become highly skilled problem solvers by appreciating generalized, system-independent approaches with many of the projects identified as high priority by NSF and DoD. Despite early successes, four main challenges remain spanning: 1) biomaterials, 2) cell sources, 3) vascularization and 4) enabling technologies such as cell delivery devices, 3D bioprinting and tissue bioreactors. REU projects will help address these challenges. WFIRM will host 10 undergraduate students over 10 summer weeks where they will engage in highly interdisciplinary and challenging areas of TERM research. Outcome objectives of the program emphasize: 1) development of interest and excitement for team-based-studies and advanced education; 2) skill transference and hands-on exposure to modern instrumentation and techniques as well as rigorous analysis; 3) enhanced verbal and written communication skills emphasizing abilities to communicate across disciplines in lay and technical terms and in formally presenting in writing (for the scientific literature) and speaking (for scientific meetings); and, ultimately, 4) to initiate socialization into the very important process of growth from dependent to independent researcher status. The REU experience is supplemented with additional program elements and education on the research process, hands-on instrumentation training, workshops on bioethics and responsible conduct of research, effective oral and written communications workshops, professional development and career opportunities topics. To increase program impact and sense of community, REU participants will also participate in non-research and outreach activities at WFIRM and with URM partners, providing forums to discuss implications of STEM advances to the larger society, while fostering networking with other students, engineers, scientists and educators. All students will present their research findings several times over the summer and in oral and poster format at a final research day event. Opportunities to attend and present at local and national meetings is also emphasized.

这个为期三年的本科生研究经验 (REU) 项目，即维克森林再生医学研究所 (WFIRM) 的工程新 REU 方法应对 MultiTERM 挑战，将在组织工程挑战性领域提供工程和生物学交叉领域的研究机会和再生医学（TERM）的对象是来自代表性不足的群体的不同本科生群体，他们使用研究设施的机会有限。拟议的研究项目涵盖多个生理系统（心血管、肌肉骨骼、泌尿系统和内分泌），为 REU 学生提供了独特的机会，通过欣赏通用的、独立于系统的方法，其中许多项目被 NSF 列为高度优先级，成为高技能的问题解决者和国防部。尽管取得了早期成功，但仍然存在四个主要挑战：1）生物材料，2）细胞来源，3）血管化和4）细胞输送设备、3D生物打印和组织生物反应器等支持技术。 REU 项目将有助于应对这些挑战。 WFIRM 将在 10 个夏季周内接待 10 名本科生，他们将参与高度跨学科且具有挑战性的 TERM 研究领域。该计划的成果目标强调：1）培养对团队学习和高等教育的兴趣和兴奋； 2) 技能传授、亲身接触现代仪器和技术以及严格的分析； 3）增强口头和书面沟通技巧，强调以通俗和技术术语进行跨学科沟通的能力以及以书面形式（科学文献）和演讲（科学会议）正式呈现的能力；最终，4）启动社会化，进入从依赖研究员身份成长为独立研究员身份的非常重要的过程。 REU 的经验还得到了额外的项目要素和研究过程教育、仪器操作培训、生物伦理学和负责任的研究行为研讨会、有效的口头和书面交流研讨会、专业发展和职业机会主题的补充。 为了提高项目影响力和社区意识，REU 参与者还将参加 WFIRM 以及 URM 合作伙伴的非研究和外展活动，提供论坛来讨论 STEM 进步对更大社会的影响，同时促进与其他学生、工程师、科学家和教育家。所有学生都将在整个夏天多次展示他们的研究成果，并在最后的研究日活动中以口头和海报的形式展示。 还强调了参加和出席地方和国家会议的机会。

项目成果

Human placental-derived stem cell therapy ameliorates experimental necrotizing enterocolitis

人胎盘干细胞疗法可改善实验性坏死性小肠结肠炎

• DOI: 10.1152/ajpgi.00369.2020
• 发表时间: 2021
• 期刊: American Journal of Physiology-Gastrointestinal and Liver Physiology
• 影响因子: 4.5
• 作者: Weis, Victoria G.;Deal, Anna C.;Mekkey, Gehad;Clouse, Cara;Gaffley, Michaela;Whitaker, Emily;Peeler, Cole B.;Weis, Jared A.;Schwartz, Marshall Z.;Atala, Anthony
• 通讯作者: Atala, Anthony

Administration of FVIII-Expressing Human Placental Cells to Juvenile Sheep Yields Multi- Organ Engraftment, Therapeutic Plasma FVIII Levels and Alter Immune Signaling Pathways to Evade FVIII Inhibitor Induction

将表达 FVIII 的人胎盘细胞给予幼年绵羊可实现多器官移植、治疗性血浆 FVIII 水平并改变免疫信号传导途径以逃避 FVIII 抑制剂诱导

• 发表时间: 2021
• 期刊: ASH
• 作者: Trevisan, Brady;Rodriguez, Martin;Dizon, Jacqueline;George, Sunil;Shields, Jordan;Lankford, Shannon;Combs, Rebessa;Owen, John;Atala, Anthony;Doering, Christopher
• 通讯作者: Doering, Christopher

Using a Human Liver Tissue Equivalent (hLTE) Platform to Define the Functional Impact of Liver-Directed AAV Gene Therapy

使用人类肝脏组织等效物 (hLTE) 平台定义肝脏定向 AAV 基因治疗的功能影响

• 发表时间: 2021
• 期刊: ASH
• 作者: Ramamurthy, R;Zheng, WT;George, S;Wan, M;Zhou, Y;Bishop, C;Atala, A;Porada, C;Almeida-Porada, G
• 通讯作者: Almeida-Porada, G

Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

• DOI: 10.1038/s41598-020-66487-8
• 发表时间: 2020-06-17
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Nzou, Goodwell;Wicks, Robert T.;Atala, Anthony J.
• 通讯作者: Atala, Anthony J.