EAGER: Developing functional gene based biomarker for DAMO and exploring the potential application of DAMO in wastewater treatment

EAGER：开发基于DAMO功能基因的生物标志物并探索DAMO在废水处理中的潜在应用

• 批准号: 1657725
• 负责人: Ramesh Goel
• 金额: $ 9.31万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1657725&HistoricalAwards=false
• 关键词: EAGER; Developing; functional; gene; based

1657725GoelAnaerobic processes are useful and enable wastewater treatment plants to become energy neutral or, possibly, energy positive. One of the useful byproducts of anaerobic processes is methane gas, which can be utilized as a fuel. This research combines two important biogeochemical cycles - the nitrogen and carbon cycles - and two important contaminants - methane and nitrogen - in surface waters. This project will contribute to solving one of the National Academy of Engineering Grand Challenges of the 21st century, managing the nitrogen cycle.Recent findings suggest that methane oxidation is possible in oxygen-free conditions with nitrite and nitrate as the electron acceptors, referred to as denitrifying anoxic methane oxidation. Denitrifying anoxic methane oxidation is ecologically significant because it connects two important ecosystem cycles - the nitrogen and carbon cycles. The role of denitrifying anoxic methane oxidation organisms cannot be ignored; these organisms contribute to the sink of methane in ecosystems as well as to the nitrogen dynamics in nitrogen-contaminated ecosystems. Recent findings related to denitrifying anoxic methane oxidation involve an addition to the biochemistry of prokaryotes-mediated methane oxidation and have generated further possibilities for research, such as an evaluation of their role on a global scale, their ecological diversity, and their actual role in methane sinks. The enzyme nitric oxide dismutase is involved in the splitting of nitric oxide (2NO=N2+O2) to generate oxygen for the particulate methane monooxygenase to oxidize methane gas. Based on the metagenome construction of M. oxyfera and preliminary stable isotope labeled experiments, it was hypothesized that M. oxyfera is capable of dismutating NO to N2 and O2. Absolutely no information is available on nitric oxide dismutase. On a broader scale, the ecological significance of denitrifying anoxic methane oxidation organisms, the actual scale of methane sinks due to denitrifying anoxic methane oxidation, and the in-situ factors affecting denitrifying anoxic methane oxidation are not fully understood. The potential of using denitrifying anoxic methane oxidation organisms for nitrogen management in wastewater treatment plants has been explored fully with well-planned operational strategies. In this EAGER award, the PI will study the role of the nitric oxide dismutase gene and determine whether studies of this gene could provide a breakthrough in understanding the ecology of denitrifying anoxic methane oxidation organisms. The PI will study the nitrogen removal potential of denitrifying anoxic methane oxidation organisms while working synergistically with other N-cycling bacteria in wastewater treatment. The intellectual merit of this research includes: (1) elucidating whether denitrification-coupled anoxic methane oxidation (denitrifying anoxic methane oxidation) is a key sink of methane in nitrogen-contaminated ecosystems, (2) revealing the identity of denitrifying anoxic methane oxidation organisms using metagenomics and metatranscriptomics, and, (3) developing a new tool to study the presence and diversity of denitrifying anoxic methane oxidation organisms. This project will lay the foundation for further investigation and incorporation of denitrifying anoxic methane oxidation in ecological models aimed at estimating methane emissions. The fundamental knowledge gained in this research will be applicable in other natural (contaminated subsurface and river sediments) and engineered (engineered bioreactors) systems. The project will directly contribute to graduate and undergraduate education. The PI has developed computer animations to demonstrate, for example, how viruses infect bacteria. In this project, the PI will further expand this outreach to include well-illustrated short films to demonstrate laboratory protocols, the significance of the nitrogen cycle, and greenhouse gas emissions. Results will be disseminated through peer-reviewed publications and community workshops for local wetland managers.

1657725 goelanaerobic过程是有用的，可以使废水处理厂成为能量中性或可能呈阳性的能量。厌氧过程的有用副产品之一是甲烷气体，可以用作燃料。这项研究结合了两个重要的生物地球化学循环 - 氮和碳循环 - 以及两个重要的污染物 - 甲烷和氮在地表水中。该项目将有助于解决21世纪美国国家工程学院的宏伟挑战之一，管理氮周期。结果表明，在无氧条件下，具有亚硝酸盐和硝酸盐作为电子受体，作为电子受体，被称为硝化甲烷氧化甲氧甲烷氧化。反硝基化缺氧甲烷氧化具有生态意义，因为它连接了两个重要的生态系统周期 - 氮和碳周期。不忽视硝化甲烷氧化生物的硝化甲烷氧化生物的作用；这些生物有助于生态系统中的甲烷下水道以及氮污染生态系统中的氮动力学。与非氧化甲烷氧化有关的最新发现涉及对原核生物介导的甲烷氧化的生物化学的增加，并为研究产生了进一步的可能性，例如评估其在全球范围内的作用，其生态多样性以及它们在甲烷水槽中的实际作用。酶一氧化氮歧义酶参与一氧化氮（2NO = N2+O2）的分裂，以生成颗粒甲烷单加氧酶的氧气以氧化甲烷气体。基于Oxyfera M. oxyfera和初步稳定的同位素标记实验的元基因组构建，假设Oxyfera M. oxyfera能够对N2和O2进行NO张开。绝对没有有关一氧化氮歧化酶的信息。在更广泛的范围内，硝化甲烷氧化生物的生态意义，由于硝化缺氧性甲烷氧化而引起的甲烷下沉的实际规模以及影响硝化甲烷氧化的硝化甲烷氧化的原位因子。使用精心计划的操作策略，充分探索了在废水处理厂中使用反硝基化甲烷氧化生物进行氮管理的潜力。在此急切的奖励中，PI将研究一氧化氮歧义酶基因的作用，并确定该基因的研究是否可以在理解硝化甲烷氧化生物的生态学方面取得突破。 PI将研究硝化甲烷氧化生物的硝化甲烷氧化生物的去除潜力，同时在废水处理中与其他N周期细菌协同作用。这项研究的智力优点包括：（1）阐明是否硝化甲烷氧化（硝化缺氧性甲烷氧化）是否是氮污染生态系统中甲烷的关键下水道研究硝化甲烷氧化生物的硝化甲烷的存在和多样性的新工具。该项目将为旨在估算甲烷排放的生态模型中进一步研究和掺入硝化甲烷氧化的基础。这项研究获得的基本知识将适用于其他天然（受污染的地下和河流沉积物）和工程（工程生物反应器）系统。该项目将直接为研究生和本科教育做出贡献。 PI开发了计算机动画，以证明病毒如何感染细菌。在该项目中，PI将进一步扩大这种外展，包括精心制作的短片，以展示实验室协议，氮循环的重要性和温室气体排放。结果将通过对当地湿地经理的同行评审出版物和社区讲习班进行传播。

项目成果

Managing dissolved methane gas in anaerobic effluents using microbial resource management-based strategies

• DOI: 10.1016/j.biortech.2019.121601
• 发表时间: 2019-10-01
• 期刊: BIORESOURCE TECHNOLOGY
• 影响因子: 11.4
• 作者: Gupta, Vedansh;Goel, Ramesh
• 通讯作者: Goel, Ramesh