Synthesis and Directed Assembly of Bio-Hybrid Materials with Membrane-Protein-Mediated Transport Performance

具有膜蛋白介导的运输性能的生物杂化材料的合成和定向组装

• 批准号: 1623241
• 负责人: Hongjun Liang
• 金额: $ 39.83万
• 依托单位: Texas Tech University Health Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1623241&HistoricalAwards=false
• 关键词: Synthesis; Directed; Assembly; Bio; Hybrid

Nontechnical: This award by the Biomaterials Program in the Division of Materials Research to Colorado School of Mines aims to overcome the materials challenge on harnessing membrane protein (MP) functions in engineered systems. MPs represent a family of biologically-derived and bio-renewable high-performance nanomaterials that are largely unexplored. These proteins are the "gate-keepers" of cells, and are involved in critical life processes, such as energy conversion, matter transport and information processing. These same MP-mediated functions are also highly coveted nanoengineering feats in synthetic systems. Exploiting MPs for nanoengineering may help understand, predict, and ultimately control recognition and transport at the nanoscale, but is greatly impeded by the fluidic and labile nature of biomembranes. This study bridges the gap between biotic and abiotic systems by developing chemically versatile synthetic membranes to support MP functions. The successful outcome of this study will help unleash the full potential of MPs to create novel nanotechnologies ranging from solar conversion to high throughput diagnostics. With respect to broad impact on education, this project builds a multi-tiered education program on renewable materials. The objective of this program is to bring societal awareness on sustainability, and motivate undergraduate and K-12 students to pursue career paths on bio-renewable materials. A focused outreach component, "Summer Experience @ Mines", targets minority students at a local high school by hosting their first exposure to engineering studies and college life, and develop curriculum materials for their Biotechnology class. A broader outreach component includes training K-12 science teachers and dissemination of the curriculum materials to local and nearby school districts. Technical: This award is to develop bio-hybrid materials with membrane-protein-mediated transport performance. Membrane proteins (MPs) are biologically-derived and bio-renewable high-performance nanomaterials. Despite numerous proof-of-concept demonstrations of MPs' great potential in engineered systems, little is known on how to design synthetic MP-supporting membranes that balance a dichotomy between fluidity and stability, and how to direct spontaneous MP reconstitution into these robust membranes to form 2-dimensional (2-D) or 3-D proteomembrane arrays. Using proteorhodopsin, a light-driven proton pump as a model, this study will elucidate: (1) the directed assembly principles to reconstitute proteorhodopsin into hierarchically organized proteomembrane arrays; and (2) the roles of synthetic membranes in shaping proteorhodopsin function. Since proteorhodopsin has a common seven transmembrane (7 TM) architecture of G protein-coupled receptors, a large family of MPs that regulate energy conversion, matter transport and biosensing. The guiding concepts learnt from this study have the potential to benefit a broad range of MP-based nanotechnologies. This multidisciplinary study provides ample opportunities to train students at the interdisciplinary area of materials engineering, synthetic chemistry, biophysics, and protein engineering. With this award, this research group will design a multi-tiered soft matter education program entitled "Renewable Materials for Sustainable Future". This program aims to: (1) improve educational components on soft matter by course development; (2) support undergraduate students from the Undergraduate Research Opportunity Program, the Society of Women Engineering, and International Exchange Students Program to have "hands-on" research experience; and (3) build regular and systematic outreach activities to K-12 students in local and nearby school districts.

非技术性：生物材料计划在科罗拉多州矿业学院的材料研究部授予该奖项，旨在克服在工程系统中利用膜蛋白（MP）功能的材料挑战。国会议员代表了一个生物学衍生和生物可再生的高性能纳米材料的家族，这些纳米材料在很大程度上没有探索。这些蛋白质是细胞的“守门员”，参与关键寿命过程，例如能量转换，物质运输和信息处理。这些相同的MP介导的功能在合成系统中也是高度令人垂涎的纳米工程功能。利用MPS进行纳米工程可能有助于理解，预测并最终控制纳米级的识别和运输，但由于生物膜的流动性和不稳定性而极大地阻碍了纳米级的识别和运输。这项研究通过开发化学多功能的合成膜来支持MP功能，从而弥合了生物和非生物系统之间的差距。这项研究的成功结果将有助于释放国会议员创建新型纳米技术的全部潜力，从太阳转换到高吞吐量诊断。关于对教育的广泛影响，该项目构建了一项关于可再生材料的多层教育计划。该计划的目的是提高对可持续性的社会意识，并激励本科生和K-12学生在可再生材料上追求职业道路。一个重点的外展部分“夏季经验 @矿山”，通过首次接触工程学和大学生活，并为其生物技术课程开发课程材料，以瞄准当地一所高中的少数民族学生。更广泛的外展部分包括培训K-12科学教师以及将课程材料传播给本地和附近的学区。 技术：该奖项旨在开发具有膜 - 蛋白质介导的运输性能的生物杂交材料。膜蛋白（MPS）是生物学衍生和可生物可再生的高性能纳米材料。 Despite numerous proof-of-concept demonstrations of MPs' great potential in engineered systems, little is known on how to design synthetic MP-supporting membranes that balance a dichotomy between fluidity and stability, and how to direct spontaneous MP reconstitution into these robust membranes to form 2-dimensional (2-D) or 3-D proteomembrane arrays.该研究将使用蛋白质淡淡的蛋白蛋白（轻驱动质子泵）作为模型，将阐明：（1）将蛋白淡淡蛋白质蛋白重新构造为层次结构化的蛋白质组膜阵列的定向装配原理； （2）合成膜在塑造蛋白淡蛋白功能中的作用。由于蛋白质淡淡蛋白酶具有G蛋白偶联受体的七个常见跨膜（7 TM）结构，这是一个调节能量转化，物质运输和生物传感的大型MP家族。从这项研究中学到的指导概念有可能受益于广泛的基于MP的纳米技术。这项多学科研究为培训材料工程，合成化学，生物物理学和蛋白质工程的跨学科领域的学生提供了足够的机会。有了这个奖项，该研究小组将设计一个多层软件教育计划，名为“可再生材料的可持续未来”。该计划的目的是：（1）根据课程开发改善有关软质的教育组成部分； （2）支持本科研究机会计划，妇女工程学会和国际交流学生计划的本科生，以拥有“动手”研究经验； （3）为当地和附近学区的K-12学生进行定期和系统的外展活动。

项目成果

Hydrophilic Phage-Mimicking Membrane Active Antimicrobials Reveal Nanostructure-Dependent Activity and Selectivity

• DOI: 10.1021/acsinfecdis.7b00076
• 发表时间: 2017-09-01
• 期刊: ACS INFECTIOUS DISEASES
• 影响因子: 5.3
• 作者: Jiang, Yunjiang;Zheng, Wan;Liang, Hongjun
• 通讯作者: Liang, Hongjun

Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers

• DOI: 10.1038/s41598-020-66852-7
• 发表时间: 2020-06-18
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Fiori, Mariana C.;Zheng, Wan;Liang, Hongjun
• 通讯作者: Liang, Hongjun

Computational and Experimental Studies of Lipid-Protein Interactions in Biomemrane Function

生物膜功能中脂质-蛋白质相互作用的计算和实验研究

• DOI: 10.1016/j.bpj.2015.11.1411
• 发表时间: 2016
• 期刊: Biophysical Journal
• 影响因子: 3.4
• 作者: Musharrafieh, Rami;Chawla, Udeep;Zheng, Wan;Kaung, Liangju;Perera, Suchithranga M.D.C.;Knowles, Thomas;Huang, Annie;Pitman, Michael C.;Wang, Jun;Liang, Hongjun
• 通讯作者: Liang, Hongjun