ERA SynBio: Orthogonal Replication as the Platform for an Episome

ERA SynBio：正交复制作为附加体的平台

• 批准号: 1545158
• 负责人: Chang Liu
• 金额: $ 58万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1545158&HistoricalAwards=false
• 关键词: ERA; SynBio; Orthogonal; Replication; Platform

This project engages 6 research groups in the United States, France and the United Kingdom. The goal is to achieve the stable maintenance and propagation of synthetic, non-natural genetic material (nucleic acids) in living cells, without integration into the genetic material of the host cell. The synthetic genetic material will have chemical features distinct from natural genetic material and will constitute a separate, independent element (called an episome) in the cell. Such a cell will sustain its growth using the natural genetic material but can make useful products when equipped with the appropriate synthetic (episomal) system in parallel. This project will generate insights into the management of chemical information in cells (including the manner in which natural systems may have evolved) and will have immediate applications in metabolic engineering. The students and young scientists working on this project will have broad and diverse international and transdisciplinary training in molecular genetics, biochemistry and bioengineering. An orthogonal bacterial episome will be developed, based on the linear plasmid system that can be stably maintained through engineered links to cellular functions and antibiotic resistance. The genetic information on the episome will be exclusively encoded by synthetic nucleic acids, termed XNAs. These XNAs have been shown to be non-toxic and unable to interact with the cellular genetic material. The international collaborators bring the complementary expertise to furnish the elements and functionalities that are necessary to build the orthogonal system. This project is funded through a transnational funding mechanism between the United States National Science Foundation and European Funding Agencies that are part of the European Commission endorsed Research Area Network in Synthetic Biology. The United States component of this project was co-funded by programs in Systems and Synthetic Biology (Directorate for Biological Sciences) and Biotechnology and Biochemical Bioengineering (Directorate for Engineering).

该项目与美国，法国和英国的6个研究小组互动。目的是实现活细胞中合成，非天然遗传物质（核酸）的稳定维持和传播，而无需整合到宿主细胞的遗传材料中。合成遗传物质的化学特征将与自然遗传物质不同，并将构成单独的独立元素（称为偶发组）。 这种细胞将使用天然遗传物质维持其生长，但在配备适当的合成（偶发性）系统时可以生产有用的产品。该项目将洞悉细胞中化学信息的管理（包括自然系统可能发展的方式），并将在代谢工程中立即应用。从事该项目的学生和年轻科学家将对分子遗传学，生物化学和生物工程进行广泛而多样的国际和跨学科培训。基于线性质粒系统，将开发正交细菌偶发组，该系统可以通过与细胞功能和抗生素耐药性的工程链接稳定地保持。有关曲线体的遗传信息将仅由称为XNA的合成核酸编码。这些XNA已被证明是无毒的，无法与细胞遗传物质相互作用。 国际合作者带来了补充专业知识，以提供建立正交系统所需的要素和功能。该项目是通过美国国家科学基金会与欧洲融资机构之间的跨国资金机制资助的，这些机制是欧盟委员会认可合成生物学研究领域网络的一部分。该项目的美国组成部分是由系统和合成生物学（生物科学局）和生物技术和生物化学生物工程（工程局）的计划共同资助的。