US-Pakistan Cooperative Research: Population Genetics of Benthosema Pterotum, A Mesopelagic Fish from the Arabian Sea

美国-巴基斯坦合作研究：阿拉伯海中层鱼类 Benthosema Pterotum 的种群遗传学

• 批准号: 0218248
• 负责人: Giacomo Bernardi
• 金额: --
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0218248&HistoricalAwards=false
• 关键词: US; Pakistan; Cooperative; Research; Population

0218248BernardiDescription: This award is for support of a joint research project between Dr. Giacomo Bernardi, Biology Department, University of California-Santa Cruz, Santa Cruz, California and Dr. Shahid Amjad, Department of Oceanography, The National Institute of Oceanography (NIO), Karachi, Pakistan. They plan to study the population genetics of Benthosema pterotum, a mesopelagic fish from the Arabian Sea. The Arabian Sea has complex oceanography, which results in high biological productivity. In the open ocean, the topmost trophic level is represented by nekton such as marine mammals and large fishes such as tunas and mackerel. In the northwest Arabian Sea and adjacent Gulf of Oman, the topmost trophic level is represented by mesopelagic fishes. These are small, midwater species such as Lanternfishes (Myctophidae), Hatchetfishes (Sternoptychidae), and Bristlemouths (Gonostomatidae). The main species reported in abundance is the lanternfish Benthosema pterotum. This will be collected off the coast of Pakistan from the Gulf of Oman to the Central Arabian Sea. Specifically, the investigators plan to investigate the genetic differences between Gulf of Oman and Arabian Sea populations of B. pterotum off the coast of Pakistan (smaller-scale). They will extract DNA, PCR amplify and sequence nuclear intron, and mitochondrial loci to establish patterns of population structure in B. pterotum. The sampling protocol will have approximately 500 individuals to have enough statistical validity. Scope: This award will allow a US team headed by the PI to collaborate with a Pakistani team from the NIO in a research project of significant scientific interest. The PIs will be working closely with young Pakistani scientists at NIO to train them for the optimum methods for fish surveys and to catch the specific species. These scientists will then carry out most of the fieldwork. They will also be trained in the new molecular techniques. Pakistan's ocean science is at a stage of development where this collaboration will greatly help. This project will have a positive impact on NIO and the fisheries departments in Pakistan. All countries bordering the northwestern Indian Ocean would like to harvest B. pterotum commercially. Improved understanding of its population genetics, and other knowledge that will follow from this pilot study, will help scientists in the region utilize this unexploited fishery resouce.

0218248Bernardi 描述：该奖项用于支持加州大学圣克鲁斯分校生物学系 Giacomo Bernardi 博士和国家海洋研究所 (NIO) 海洋学系 Shahid Amjad 博士之间的联合研究项目，巴基斯坦卡拉奇。 他们计划研究 Benthosema pterotum（一种来自阿拉伯海的中层鱼类）的种群遗传学。 阿拉伯海海洋学复杂，生物生产力高。 在公海，最顶层的营养级以海洋哺乳动物等游生物和金枪鱼和鲭鱼等大型鱼类为代表。 在阿拉伯海西北部和邻近的阿曼湾，最高营养级以中层鱼类为代表。 这些是小型中层鱼类，如灯笼鱼（Myctophidae）、斧头鱼（Sternoptychidae）和刚口鱼（Gonostomatidae）。 据报道，数量最多的主要物种是灯笼鱼 Benthosema pterotum。 这些将在巴基斯坦海岸从阿曼湾到阿拉伯海中部收集。 具体来说，研究人员计划调查巴基斯坦海岸附近阿曼湾和阿拉伯海的翼翅双歧杆菌种群之间的遗传差异（规模较小）。 他们将提取 DNA、PCR 扩增并对核内含子和线粒体基因座进行测序，以建立翼芽孢杆菌群体结构模式。 抽样方案将有大约 500 名个体，以具有足够的统计有效性。范围：该奖项将允许由 PI 领导的美国团队与 NIO 的巴基斯坦团队合作开展具有重大科学意义的研究项目。 PI 将与 NIO 的年轻巴基斯坦科学家密切合作，培训他们掌握鱼类调查和捕获特定物种的最佳方法。然后，这些科学家将进行大部分实地工作。他们还将接受新分子技术的培训。 巴基斯坦海洋科学正处于发展阶段，此次合作将大有裨益。 该项目将对蔚来汽车和巴基斯坦渔业部门产生积极影响。 所有与西北印度洋接壤的国家都希望商业化捕捞翼藻。 更好地了解其种群遗传学以及这项试点研究得出的其他知识，将有助于该地区的科学家利用这种未开发的渔业资源。