MRI: Upgrade of an EPR Spectrometer to Serve Marquette University and the Greater Milwaukee Region

MRI：升级 EPR 光谱仪以服务马凯特大学和大密尔沃基地区

• 批准号: 1532168
• 负责人: Brian Bennett
• 金额: $ 16.28万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1532168&HistoricalAwards=false
• 关键词: MRI; Upgrade; EPR; Spectrometer; Serve

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF), Professors Brian Bennett and colleague Richard Holz from Marquette University will upgrade of an electron paramagnetic resonance (EPR) spectrometer. This upgrade will provide a high-quality cryogenic EPR system for research and education, without the expense of a new instrument. This equipment will allow research in a variety of fields such as those that provide insight on how biologically relevant species with unpaired electrons behave. In general, an EPR spectrometer yields detailed information on the geometric and electronic structure of molecular and solid state materials. It may also be used to obtain information about the lifetimes of free radicals, short-lived, highly reactive species involved in valuable chemical transformations as well as the initiation of pathological tumor growth. These studies will impact a number of areas, from the synthesis of inorganic and organic molecules to the development of new solid state materials to compounds of magnetic and biological interest. Employing examples inspired from ongoing research, this instrument will be an integral part of research and teaching at the undergraduate and graduate levels at Marquette. Additionally, the investigators have identified a significant number of interested users from the area outside of this institution. Examples are major users from the Universities of Wisconsin at Milwaukee, Madison and Parkside as well as St Maurice College, Milwaukee School of Engineering and Concordia University.The award is aimed at enhancing research and education at all levels, especially in areas such as (a) carrying out mechanistic studies on nitrile hydratase; (b) studying structural and functional models for metalloenzyme active centers; (c) studying inorganic supramolecular and materials chemistry; (d) studying nitrogen-cycle enzymes; (e) assembling and regulating of Cyt c oxidase by Hig1 and oxa1p; and (f) investigating carbenes, carbocations and non-covalent halogen interactions.

凭借主要研究仪器计划 (MRI) 的奖项和化学研究仪器计划 (CRIF) 的支持，马凯特大学的 Brian Bennett 教授和同事 Richard Holz 将升级电子顺磁共振 (EPR) 波谱仪。此次升级将为研究和教育提供高质量的低温 EPR 系统，而无需购买新仪器。该设备将允许在各个领域进行研究，例如那些提供有关具有不成对电子的生物学相关物种如何行为的见解的领域。一般来说，EPR 光谱仪可提供分子和固态材料的几何和电子结构的详细信息。它还可用于获取有关自由​​基、短命、高活性物质的寿命的信息，这些物质参与有价值的化学转化以及病理性肿瘤生长的启动。这些研究将影响许多领域，从无机和有机分子的合成到新型固态材料的开发，再到具有磁性和生物意义的化合物。该仪器采用受正在进行的研究启发的示例，将成为马凯特大学本科生和研究生研究和教学的一个组成部分。此外，调查人员还从该机构以外的地区识别出大量感兴趣的用户。例如，来自威斯康星大学密尔沃基分校、麦迪逊分校和 Parkside 大学以及圣莫里斯学院、密尔沃基工程学院和康考迪亚大学的主要用户。该奖项旨在加强各级的研究和教育，特别是在以下领域： ) 进行腈水合酶的机理研究； (b) 研究金属酶活性中心的结构和功能模型； (c) 研究无机超分子和材料化学； (d) 研究氮循环酶； (e) Hig1和oxa1p对Cyt c氧化酶的组装和调节； (f) 研究卡宾、碳阳离子和非共价卤素相互作用。

项目成果

Cellular maturation of an iron-type nitrile hydratase interrogated using EPR spectroscopy

使用 EPR 光谱研究铁型腈水合酶的细胞成熟

• DOI: 10.1007/s00775-019-01720-y
• 发表时间: 2019-10
• 期刊: JBIC Journal of Biological Inorganic Chemistry
• 作者: Lankathilaka, K. P.;Stein, Natalia;Holz, Richard C.;Bennett, Brian
• 通讯作者: Bennett, Brian

Analyzing the function of the insert region found between the α and β-subunits in the eukaryotic nitrile hydratase from Monosiga brevicollis

分析 Monosiga brevicollis 真核腈水合酶中 α 和 β 亚基之间插入区域的功能

• DOI: 10.1016/j.abb.2018.08.013
• 发表时间: 2018-11
• 期刊: Archives of Biochemistry and Biophysics
• 影响因子: 3.9
• 作者: Yang, Xinhang;Bennett, Brian;Holz, Richard C.
• 通讯作者: Holz, Richard C.

Identification of an Intermediate Species along the Nitrile Hydratase Reaction Pathway by EPR Spectroscopy

通过 EPR 光谱鉴定腈水合酶反应途径的中间物质

• DOI: 10.1021/acs.biochem.1c00574
• 发表时间: 2021-12-14
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Karunagala Pathiranage WL;Gumataotao N;Fiedler AT;Holz RC;Bennett B
• 通讯作者: Bennett B