SBIR Phase II: Development of a Microscope to Detect Cellular Motion in Three-dimensional Tissue

SBIR 第二阶段：开发用于检测三维组织中细胞运动的显微镜

• 批准号: 1534699
• 负责人: Ran An
• 金额: $ 74.41万
• 依托单位: Animated Dynamics, Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1534699&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Development; Microscope

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project is to develop a new microscope that integrates biodynamic imaging with conventional phase contrast microscopy for use in drug development and other research applications. Accurately capturing the signatures of three-dimensional biological systems provides more realistic measures of drug efficacy and response heterogeneity - two critical aspects of the drug-development process that help find the right drug doses and combinations to kill cancer cells with minimal toxic side effects. This tool will extract functional information at greater tissue depths than conventional microscopies by using cellular motions inside living tissue. The motion is detected by reflected light that carries with it important information about the action of drugs inside 3D tissue, allowing drug evaluations in heterogeneous three-dimensional tissues systems, and providing new insights for pharmaceutical development. This SBIR Phase II project provides a tool capable of extracting high-content information from inside three-dimensional living tissue culture and tumor biopsies. It extracts this functional information at greater tissue depths than conventional microscopies by using Doppler tags on light scattered from intracellular motions inside living tissue. These novel Doppler tags carry phenotypic profiling information about the action of drugs inside 3D tissue, allowing drug evaluations in heterogeneous three-dimensional tissues and providing new insights for pharmaceutical development and therapeutics. The project goals are to construct a new type of microscope based on the detection of cellular motions, and to integrate the microscope with a software platform that is easy to use by customers. Upon completion of this Phase II, a commercial biodynamic microscope product will be ready for value-added resale of leading microscopes to life sciences customers.

这项小型企业创新研究（SBIR）II期项目的更广泛的影响/商业潜力是开发一种新的显微镜，将生物动力成像与常规相对比度显微镜整合到药物开发和其他研究应用中。 准确捕获三维生物系统的特征提供了更现实的药物功效和反应异质性的衡量标准 - 药物开发过程的两个关键方面，有助于找到正确的药物剂量和组合，以杀死具有最小有毒副作用的癌细胞。通过使用活组织内部的细胞运动，该工具将比传统显微镜在组织深度更大的组织深度提取功能信息。 通过反射光检测到该运动，它带有有关3D组织中药物作用的重要信息，从而可以在异质的三维组织系统中进行药物评估，并为药物开发提供新的见解。 该SBIR II期项目提供了一种工具，能够从三维生活组织培养和肿瘤活检中提取高内心信息。它通过使用多普勒标签在活组织内细胞内运动的光上使用多普勒标签来提取比常规显微镜更大的组织深度中提取这些功能信息。 这些新型的多普勒标签具有有关3D组织中药物作用的表型分析信息，从而可以在异质三维组织中进行药物评估，并为药物开发和治疗剂提供新的见解。 项目目标是基于蜂窝动作的检测来构建一种新型的显微镜，并将显微镜与客户易于使用的软件平台集成。 II阶段完成后，商业生物动力显微镜产品将准备好将带向生命科学客户的领先显微镜转售的增值转售。