Peptide Derivatized Poly(ester urea)s for Regenerative Medicine

用于再生医学的肽衍生聚（酯脲）

• 批准号: 1507420
• 负责人: Matthew Becker
• 金额: $ 39万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1507420&HistoricalAwards=false
• 关键词: Peptide; Derivatized; Poly; ester; urea

Nontechnical: This award by the Biomaterials program in the Division of Materials Research to the University of Akron aims at discovering systematic method for controllably tethering bioactive peptides to novel polymer scaffolds for use in regenerative medicine applications. This award is also co-funded by the Biomedical Engineering program in CBET/ENG. If successful, this work will optimize peptide concentrations that enhance cell proliferation and accelerate stem cell differentiation. This knowledge would help guide the rational design of future peptide-functionalized polymer surfaces, scaffolds and hydrogels used by the wider tissue engineering community. The biological measurements will drive the design of advanced bioactive and biomimetic materials for use in many applications where synthetic materials contact biological systems. The research team is involved in the educational training components of the project. The effort will provide advanced, multidisciplinary training to undergraduate and graduate students, orthopedic surgery residents, and high school students in areas of molecular and cell biology, chemistry and polymer science. The outcomes from the research are being directly incorporated into graduate level and undergraduate courses being taught by Professor Becker. Undergraduate students from the University of Akron, and summer interns enrolled in the research experiences for undergraduates (REU) program will participate extensively. The research team's outreach activities include mentoring and provide educational and research experience for students from local high schools.Technical: This award by the Biomaterials program in the Division of Materials Research and the Biomedical Engineering program in CBET/ENG to the University of Akron aims at discovering a systematic method for controllably tethering multiple bioactive peptides to novel poly(ester urea) scaffolds post-3D printing for use in regenerative medicine applications. The biomaterials community clearly understands that the molecular presentation and spatial distribution of peptides and growth factors can dramatically influence many important aspects of cell behavior. This award aims to translate previously discovered GRGDS and BMP-2 peptide concentrations to translationally-relevant degradable poly(ester urea) constructs in two-dimensional thin films and three-dimensional printed scaffolds. The project will impact several fields of biomaterials including (1) developing new methods for functionalization of degradable polymer scaffolds with bioactive peptides post 3D printing and (2) new methods for characterizing surface concentrations of these peptides and (3) identification of synergistic peptide concentrations that influence hMSC cell proliferation, lineage commitment, and differentiation. The project offers multiple opportunities for the educational training of students (both undergraduate and graduate) and medical residents in advanced polymer science, state of the art chemical functionalization and translationally-relevant strategies for advanced manufacturing of 3D scaffolds. These skills will be critical to public and private entities aiming to move 3D printed scaffolds into mainstream device and regenerative medicine applications.

非技术性：该奖项由阿克伦大学材料研究部生物材料项目颁发，旨在发现将生物活性肽可控地束缚到新型聚合物支架上的系统方法，用于再生医学应用。该奖项还由 CBET/ENG 生物医学工程项目共同资助。如果成功，这项工作将优化肽浓度，从而增强细胞增殖并加速干细胞分化。 这些知识将有助于指导更广泛的组织工程界使用的未来肽功能化聚合物表面、支架和水凝胶的合理设计。生物测量将推动先进生物活性和仿生材料的设计，用于合成材料接触生物系统的许多应用。 研究团队参与该项目的教育培训部分。这项工作将为本科生和研究生、骨科住院医生和高中生提供分子和细胞生物学、化学和聚合物科学领域的高级、多学科培训。 研究成果直接纳入贝克尔教授教授的研究生和本科生课程中。 阿克伦大学的本科生和参加本科生研究体验（REU）项目的暑期实习生将广泛参与。研究团队的外展活动包括为当地高中的学生提供指导并提供教育和研究经验。技术：该奖项由材料研究部的生物材料项目和 CBET/ENG 的生物医学工程项目授予阿克伦大学，旨在发现一种系统方法，可在 3D 打印后将多种生物活性肽可控地束缚到新型聚（酯脲）支架上，用于再生医学应用。 生物材料界清楚地认识到，肽和生长因子的分子呈现和空间分布可以极大地影响细胞行为的许多重要方面。该奖项旨在将先前发现的 GRGDS 和 BMP-2 肽浓度转化为二维薄膜和三维打印支架中与翻译相关的可降解聚（酯脲）结构。 该项目将影响生物材料的多个领域，包括（1）开发3D打印后用生物活性肽功能化可降解聚合物支架的新方法，以及（2）表征这些肽表面浓度的新方法，以及（3）识别协同肽浓度影响 hMSC 细胞增殖、谱系定型和分化。 该项目为学生（本科生和研究生）和住院医师提供多种教育培训机会，涉及先进聚合物科学、最先进的化学功能化以及 3D 支架先进制造的转化相关策略。 这些技能对于旨在将 3D 打印支架转移到主流设备和再生医学应用的公共和私人实体至关重要。