MRI: ACQUISITION OF AN IMAGING SURFACE PLASMON RESONANCE SPECTROMETER FOR QUANTITATIVE ASSESSMENT OF SURFACE ADSORBING SPECIES

MRI：获取成像表面等离激元共振光谱仪，用于表面吸附物质的定量评估

• 批准号: 1126544
• 负责人: Matthew Becker
• 金额: $ 30万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1126544&HistoricalAwards=false
• 关键词: MRI; ACQUISITION; IMAGING; SURFACE; PLASMON

Technical AbstractSurface plasmon resonance (SPR) is an evanescent wave refractive index measurement made at a metal-coated surface and has been demonstrated to be a quantitative, sensitive, and label-free technique for measuring the binding kinetics of proteins, DNA, cells, and small molecules to surface-immobilized capture agents. The combination of imaging with SPR (SPRI) allows sensitive and accurate quantification of cell areas and dynamic changes in cell-substrate interactions such as membrane ruffling. SPRI also affords quantification of secretion of proteins from cells, and changes in surface protein density as a function of time and location. The custom-designed SPRI will aid researchers in studying adsorption of peptides, nanoparticles, proteins and other bioconjugates on polymers, self assembled monolayers and even live cells in culture. The fluorescence microscopy configuration will enable the capture of cell morphology information, spatial distributions of ligands and receptors and competitive molecular uptake studies which when coupled to the adsorption and kinetic data from SPRi will provide opportunities for quantitative and statistical measurements of cell material interactions. In 2009, Dr. Becker initiated a long-term outreach effort with St. Vincent / St. Mary High School (STVM). It has grown to include 10 additional faculty members at UA. Uniquely, EVERY year, at each grade level, EVERY student is required to complete a year-long, inquiry-based science project as part of the science curriculum. The science inquiry method encourages students to ask in depth questions and then to apply themselves, including conducting experiments, to find the answers, rather than merely memorize facts. The students research and identify a problem that they proceed to solve or answer by carefully and methodically performing experiments, conducting research and consulting with the teams of faculty, graduate students, high school teachers and students. Results are formulated into ACS POLYMER PREPRINT style manuscripts. Collectively the efforts of 11 teams (student, faculty, teacher) led to STVM in winning the Harold C. Shaw Outstanding School Award and named as the outstanding high school science program in the State of Ohio in both 2010 and 2011. We aim to use real data collected by the students in the science inquiry program to demonstrate physical phenomena of refractive index, optical adsorption and protein adsorption which will be incorporated into new modules in the high school physics curriculum.Non-Technical AbstractThe interactions of molecules with surfaces play an important role in many biological and physical processes. The adsorption of these biomolecules are important for designing materials that have antimicrobial properties, developing scaffolds for tissue engineering, bone growth, and drug delivery. The acquisition of Imaging Surface Plasmon Resonance Instrumentation (SPR) will provide information on adsorption of proteins, peptides and nanometer-size particles on surfaces. This new capability at the University of Akron (UA) will provide opportunities for students and faculty members in twelve research groups to investigate fundamental and applied problems in the area of biomaterials. This additional capability will accelerate the development of new biomaterials and provide important training to graduate and undergraduate students. In addition local high school students participating in a year-long science inquiry program at UA will use the instrumentation to study kinetics of adsorption. We aim to use real data collected by the students to demonstrate physical phenomena of refractive index, optical adsorption and protein adsorption which will be incorporated into new modules in the high school physics curriculum.

技术摘要表面等离子体共振（SPR）是一种在金属涂层表面进行的逃生波折射率测量，已被证明是一种定量，敏感且无标记的技术，用于测量蛋白质，DNA，DNA，细胞和小分子至表面捕获的捕获的蛋白质的结合动力学。成像与SPR（SPRI）的组合允许对细胞区域的敏感，准确的定量以及细胞基底相互作用的动态变化，例如膜褶皱。 SPRI还提供了对细胞中蛋白质分泌的定量，表面蛋白质密度随时间和位置的函数的变化。定制设计的SPRI将帮助研究人员研究聚合物上的肽，纳米颗粒，蛋白质和其他生物缀合物的吸附，自组装单层，甚至在培养中活细胞。 荧光显微镜构型将使捕获细胞形态信息，配体和受体的空间分布以及竞争性的分子摄取研究，当与SPRI的吸附和动力学数据耦合时，将为细胞材料相互作用的定量和统计测量提供机会。 2009年，贝克尔博士与圣文森特 /圣玛丽高中（STVM）进行了长期的外展工作。 它已经成长为在UA的其他10位教职员工。独特的是，每年在每个年级上，每个学生都必须完成一个为期一年的基于询问的科学项目，作为科学课程的一部分。 科学探究方法鼓励学生提出深入的问题，然后应用自己，包括进行实验，以找到答案，而不仅仅是记住事实。 学生研究并确定了他们通过仔细而有条不紊地进行实验，与教职员工，研究生，高中老师和学生进行研究和咨询来解决或回答的问题。 结果被配制为ACS聚合物预印本手稿。共有11个团队（学生，教师，老师）的努力导致STVM赢得了Harold C. Shaw杰出学校奖，并在2010年和2011年赢得了俄亥俄州杰出的高中科学计划。我们旨在使用学生在科学探究计划中收集的真实数据来证明物理学的现象，以展示出色的型号，将其用于新模式，将其用于新型的质量吸收和protialtial Adsore，这将融入该模型，并将其融合在一起课程。非技术摘要分子与表面的相互作用在许多生物学和物理过程中起重要作用。这些生物分子的吸附对于设计具有抗菌特性的材料，开发用于组织工程，骨骼生长和药物输送的脚手架很重要。成像表面等离子体共振仪（SPR）的采集将提供有关表面上蛋白质，肽和纳米尺寸颗粒吸附的信息。阿克伦大学（UA）的新能力将为十二个研究小组的学生和教职员工提供机会，以调查生物材料领域的基本问题和应用问题。这种额外的能力将加快新生物材料的发展，并为研究生和本科生提供重要的培训。 此外，参加UA的长达一年科学探究计划的当地高中生将使用该仪器研究吸附动力学。 我们的目标是使用学生收集的实际数据来展示折射率，光吸附和蛋白质吸附的物理现象，这些现象将纳入高中物理课程的新模块中。