MRI: ACQUISITION OF AN IMAGING SURFACE PLASMON RESONANCE SPECTROMETER FOR QUANTITATIVE ASSESSMENT OF SURFACE ADSORBING SPECIES

MRI：获取成像表面等离激元共振光谱仪，用于表面吸附物质的定量评估

• 批准号: 1126544
• 负责人: Matthew Becker
• 金额: $ 30万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1126544&HistoricalAwards=false
• 关键词: MRI; ACQUISITION; IMAGING; SURFACE; PLASMON

Technical AbstractSurface plasmon resonance (SPR) is an evanescent wave refractive index measurement made at a metal-coated surface and has been demonstrated to be a quantitative, sensitive, and label-free technique for measuring the binding kinetics of proteins, DNA, cells, and small molecules to surface-immobilized capture agents. The combination of imaging with SPR (SPRI) allows sensitive and accurate quantification of cell areas and dynamic changes in cell-substrate interactions such as membrane ruffling. SPRI also affords quantification of secretion of proteins from cells, and changes in surface protein density as a function of time and location. The custom-designed SPRI will aid researchers in studying adsorption of peptides, nanoparticles, proteins and other bioconjugates on polymers, self assembled monolayers and even live cells in culture. The fluorescence microscopy configuration will enable the capture of cell morphology information, spatial distributions of ligands and receptors and competitive molecular uptake studies which when coupled to the adsorption and kinetic data from SPRi will provide opportunities for quantitative and statistical measurements of cell material interactions. In 2009, Dr. Becker initiated a long-term outreach effort with St. Vincent / St. Mary High School (STVM). It has grown to include 10 additional faculty members at UA. Uniquely, EVERY year, at each grade level, EVERY student is required to complete a year-long, inquiry-based science project as part of the science curriculum. The science inquiry method encourages students to ask in depth questions and then to apply themselves, including conducting experiments, to find the answers, rather than merely memorize facts. The students research and identify a problem that they proceed to solve or answer by carefully and methodically performing experiments, conducting research and consulting with the teams of faculty, graduate students, high school teachers and students. Results are formulated into ACS POLYMER PREPRINT style manuscripts. Collectively the efforts of 11 teams (student, faculty, teacher) led to STVM in winning the Harold C. Shaw Outstanding School Award and named as the outstanding high school science program in the State of Ohio in both 2010 and 2011. We aim to use real data collected by the students in the science inquiry program to demonstrate physical phenomena of refractive index, optical adsorption and protein adsorption which will be incorporated into new modules in the high school physics curriculum.Non-Technical AbstractThe interactions of molecules with surfaces play an important role in many biological and physical processes. The adsorption of these biomolecules are important for designing materials that have antimicrobial properties, developing scaffolds for tissue engineering, bone growth, and drug delivery. The acquisition of Imaging Surface Plasmon Resonance Instrumentation (SPR) will provide information on adsorption of proteins, peptides and nanometer-size particles on surfaces. This new capability at the University of Akron (UA) will provide opportunities for students and faculty members in twelve research groups to investigate fundamental and applied problems in the area of biomaterials. This additional capability will accelerate the development of new biomaterials and provide important training to graduate and undergraduate students. In addition local high school students participating in a year-long science inquiry program at UA will use the instrumentation to study kinetics of adsorption. We aim to use real data collected by the students to demonstrate physical phenomena of refractive index, optical adsorption and protein adsorption which will be incorporated into new modules in the high school physics curriculum.

技术摘要表面等离子体共振 (SPR) 是一种在金属涂层表面进行的倏逝波折射率测量，已被证明是一种定量、灵敏且无标记的技术，用于测量蛋白质、DNA、细胞和蛋白质的结合动力学。小分子到表面固定的捕获剂。成像与 SPR (SPRI) 相结合，可以灵敏、准确地量化细胞面积以及细胞与基质相互作用的动态变化，例如膜皱褶。 SPRI 还可以定量细胞蛋白质的分泌，以及表面蛋白质密度随时间和位置的变化。定制设计的 SPRI 将帮助研究人员研究肽、纳米粒子、蛋白质和其他生物结合物在聚合物、自组装单分子层甚至培养物中活细胞上的吸附。 荧光显微镜配置将能够捕获细胞形态信息、配体和受体的空间分布以及竞争性分子摄取研究，当与 SPRi 的吸附和动力学数据结合时，将为细胞材料相互作用的定量和统计测量提供机会。 2009 年，贝克尔博士发起了与圣文森特/圣玛丽高中 (STVM) 的长期外展工作。 UA 的教职人员现已增加到 10 名。独特的是，每年每个年级的每个学生都必须完成为期一年的基于探究的科学项目，作为科学课程的一部分。 科学探究方法鼓励学生提出深入的问题，然后通过自己的实践，包括进行实验来寻找答案，而不是仅仅记住事实。 学生们通过仔细、有条理地进行实验、与教师、研究生、高中教师和学生团队进行研究和咨询，研究并确定他们要解决或回答的问题。 结果被制定为 ACS POLYMER PREPRINT 风格的手稿。 11 个团队（学生、教师、教师）的共同努力使 STVM 赢得了 Harold C. Shaw 杰出学校奖，并于 2010 年和 2011 年被评为俄亥俄州杰出高中科学项目。我们的目标是利用学生在科学探究项目中收集的真实数据，用于演示折射率、光学吸附和蛋白质吸附的物理现象，这些物理现象将被纳入高中物理课程的新模块中。非技术摘要分子与表面的相互作用在许多生物和物理过程中发挥着重要作用。这些生物分子的吸附对于设计具有抗菌特性的材料、开发组织工程、骨骼生长和药物输送的支架非常重要。收购成像表面等离子共振仪器（SPR）将提供有关表面上蛋白质、肽和纳米尺寸颗粒吸附的信息。阿克伦大学 (UA) 的这一新能力将为十二个研究小组的学生和教师提供研究生物材料领域的基础和应用问题的机会。这种额外的能力将加速新型生物材料的开发，并为研究生和本科生提供重要的培训。 此外，参加 UA 为期一年的科学探究项目的当地高中生将使用该仪器来研究吸附动力学。 我们的目标是利用学生收集的真实数据来演示折射率、光学吸附和蛋白质吸附的物理现象，这些物理现象将被纳入高中物理课程的新模块中。