Photochemical Strategies with Supramolecular Assistance to Monitor Cellular Dynamics in Living Organism

超分子辅助光化学策略监测活体细胞动力学

• 批准号: 1505885
• 负责人: Francisco Raymo
• 金额: $ 45万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-01-15 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1505885&HistoricalAwards=false
• 关键词: Photochemical; Strategies; Supramolecular; Assistance; Monitor

This project aims at the design and preparation of molecules that can be transported to target locations in organisms and emit light only after stimulation with an activating optical beam. Subsequent detection of the emitted light offers the opportunity to locate the position of the activated molecules in a sample of interest and follow their movements in real time. These light emitters can ultimately become analytical tools for biological studies, such as the tracking of moving cells during the growth of the organism, and contribute valuable insights on organismal development. These fundamental studies in chemistry may have implications in biology and medicine. Additionally, the activities teach the participating undergraduate and graduate students how to prepare molecules from commercial reagents, characterize their structures and investigate their interactions with light. Furthermore, they contribute to strengthening an existing educational collaboration between the laboratory of the principal investigator at the University of Miami and a faculty member of Miami-Dade College. This collaboration exposes high-school and undergraduate students to research. Considering that both institutions have minority enrollments in excess of 50%, these training opportunities may have a significant educational impact on members of underrepresented groups.The goal of this research is the development of biocompatible probes with photoactivatable fluorescence in the deep-red and near-infrared regions of the electromagnetic spectrum. Specifically, this project involves the synthesis of molecules incorporating a fluorescent borondipyrromethene chromophore and a photoswitchable oxazine auxochrome, together with their photochemical/photophysical characterization and incorporation within polymer nanoparticles. Upon illumination at an appropriate activation wavelength, the photoinduced and irreversible opening of the oxazine heterocycle of these compounds is expected to extend the electronic conjugation of the adjacent borondipyrromethene chromophore. This structural transformation is designed to bathochromically shift the main absorption band of the latter component and allow its selective excitation at a suitable wavelength with concomitant fluorescence. On the basis of these operating principles, the translocation of the photochemical and emissive product, across a given sample of interest, can then be probed in real time with the sequential acquisition of fluorescence images. These light emitters can ultimately become analytical tools for biological studies, such as the tracking of moving cells during the growth of the organism, and contribute valuable insights on organismal development.

该项目旨在设计和制备分子，这些分子可以将其运输到生物体中的靶位置，并仅在激活光束刺激后发出光。 随后对发射光的检测提供了机会，可以在感兴趣的样本中定位活化分子的位置，并实时跟随其运动。 这些光发射器最终可以成为生物学研究的分析工具，例如在生长过程中对移动细胞的跟踪，并为有机体发育提供宝贵的见解。 这些化学方面的基本研究可能对生物学和医学有影响。 此外，这些活动教会参与的本科生和研究生如何从商业试剂中制备分子，对其结构进行特征并研究其与光的相互作用。 此外，他们有助于加强迈阿密大学首席研究员实验室与迈阿密戴德学院的教职员工之间的现有教育合作。 这种合作使高中生和本科生进行了研究。 考虑到这两个机构的少数入学人数超过50％，这些培训机会可能会对代表性不足的群体的成员产生重大的教育影响。这项研究的目的是发展具有光活化荧光的生物相容性概率在深红色和接近 - 电磁频谱的红外区域。 具体而言，该项目涉及合成掺入荧光硼吡啶甲烯发色团的分子和可照相机的可替嗪酸的选择性的选择，以及它们的光化学/光学特征和聚合物纳米颗粒中的掺入。 在适当的激活波长下照明后，预计这些化合物的羟嗪杂环的光诱导和不可逆的开放将延伸相邻的硼迪吡喃甲烷发色团的电子结合。 这种结构转化旨在从浸泡物上移动后一种组件的主要吸收带，并在适当的波长和伴随荧光的合适波长下进行选择性激发。 基于这些操作原则，可以通过顺序获取荧光图像实时探测光化学和发射产品的易位。 这些光发射器最终可以成为生物学研究的分析工具，例如在生长过程中对移动细胞的跟踪，并为有机体发育提供宝贵的见解。