DISSERTATION RESEARCH: Evolutionary Trends Within the Polemoniaceae (Ericales)

论文研究：杜鹃花科（Ericales）的进化趋势

• 批准号: 1501867
• 负责人: Kenneth Sytsma
• 金额: $ 1.71万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1501867&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Evolutionary; Trends; Within

The Polemoniaceae, the phlox family, is a textbook example of an adaptive radiation and has long been studied by biologists from diverse fields under the assumption that pollinators have played a key role in the formation of its species. However, this, hypothesis remains untested. This research explores evolution in the phlox family, a group of plants with most species found in the United States to test this this hypotheses from ecological and genetic perspectives. This research will benefit science and society by developing identification keys which will allow the public, especially members of native plant organizations, to identify and therefore have a deeper appreciation for the biological diversity of phlox. These data will also allow conservationists to make informed, cost-effective decisions as they seek to preserve endangered and threatened species at both the State and Federal level, especially in California, Nevada, Minnesota, and Wisconsin.Polemonium has been the subject of multiple molecular phylogenetic studies yet the relationships among its species remain undetermined. Species boundaries between species are also unclear, perhaps as a result of reticulate evolution. This project will generate a near species-level phylogeny of the Polemoniaceae and will also use morphometric techniques to generate trait data. This study will be the first to sample all species and utilize multiple nuclear loci to determine species relationships. These data will test the hypothesis that floral form has been selected for by pollinators and the morphometric data will determine the evolution of leaf form. Collection data from natural history museums will be used to investigate the biogeographic history of Polemonium. The use of comparative phylogenetic methods with these data sets provides a unique opportunity to reconstruct the ancestral character states at various time periods during phlox evolution. Polemonium occidentale ssp. lacustre is endangered in both states in which it occurs, but is not yet Federally listed as endangered. Next-generation sequencing approaches will be used to determine its taxonomic status and population structure to inform conservation of this rare taxon.

Polemoniaceae是phlox家族，是适应性辐射的教科书示例，长期以来，由不同领域的生物学家研究了授粉媒介在其物种形成中起着关键作用的假设。但是，这是未经测试的假设。这项研究探索了phlox家族的进化，这是一组在美国发现的植物，这些植物是从生态和遗传学的角度来检验这一点的。 这项研究将通过开发识别密钥来使科学和社会受益，这将使公众，特别是本地植物组织的成员识别，因此对phlox的生物学多样性有了更深刻的认识。 这些数据还将允许保护主义者在寻求在州和联邦一级保持濒危和受威胁的物种时，尤其是在加利福尼亚，内华达州，明尼苏达州和威斯康星州。Polemonium一直是多个分子系统生理研究的主题，但其物种之间的关系仍未确定。物种之间的物种边界也不清楚，这可能是由于网状进化而导致的。该项目将产生近似物种水平的系统发育，并将使用形态计量技术生成性状数据。 这项研究将是第一个采样所有物种并利用多个核基因座来确定物种关系的研究。这些数据将检验以授粉媒介选择花卉形式的假设，形态学数据将决定叶片形式的演变。 自然历史博物馆的收集数据将用于研究polemonium的生物地理历史。 与这些数据集的比较系统发育方法的使用为在phlox进化过程中的不同时间段内重建祖先特征状态提供了独特的机会。 POLMONIUM OUCCIDENDALE SSP。 Lacustre在两种情况下都濒临灭绝，但尚未将联邦列为濒危。 下一代测序方法将用于确定其分类学状态和人口结构，以告知这种罕见分类单元的保护。