Role of L-type Ca2+-channels for development and function of the medial nucleus of the trapezoid body, an ultrafast relay center involved in sound localization

L 型 Ca2 通道对梯形体内侧核发育和功能的作用，梯形体内侧核是参与声音定位的超快中继中心

• 批准号: 218321014
• 负责人: Professor Dr. Eckhard Friauf
• 金额: --
• 依托单位: Fachgebiet Tierphysiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/218321014?language=en
• 关键词: Role; type; Ca2+; channels; development

A hallmark of sound localization circuits in the mammalian auditory brainstem is ultrafast, secure and faithful neurotransmission. The medial nucleus of the trapezoid body (MNTB) takes an important part in these circuits as a temporally highly precise relay station. MNTB neurons invert excitatory input from the contralateral ear to inhibitory inputs to the ipsilateral lateral superior olive and the medial superior olive, two binaural centers, processing interaural level and time differences. The molecular mechanisms underpinning correct formation and function of these sound localization circuits are poorly understood. Own data identified a critical role of the L-type Ca2+-channels Cav1.2 and Cav1.3 in these processes. Here, we propose to unravel the precise function of these two channels in the MNTB. We will address both the functional consequences of auditory brainstem-specific loss of these two channels as well as their precise role for development and maturation of MNTB neurons. This will be done by applying state-of-the-art techniques on the genetic, molecular, cellular, and electrophysiological level up to the systems level. Taken all data together will provide important new insight into molecular mechanisms involved in the generation of unique specializations of the auditory system.

哺乳动物听觉脑干中声音定位电路的标志是超快，安全和忠实的神经传递。梯形体（MNTB）的内侧核在这些电路中作为时间高度精确的继电器站起来。 MNTB神经元将兴奋性输入从对侧耳朵到抑制性输入到同侧侧向上橄榄和内侧上橄榄，两个双耳中心，处理室内水平和时间差异。这些声音定位电路的正确形成和功能的分子机制知之甚少。自己的数据在这些过程中确定了L型Ca2+通道CAV1.2和CAV1.3的关键作用。在这里，我们建议揭示MNTB中这两个通道的精确功能。我们将解决这两个通道的听觉脑干特异性丢失的功能后果，又要解决它们在MNTB神经元发展和成熟的确切作用。这将通过将最新技术应用于遗传，分子，细胞和电生理水平上的最新技术，直到系统水平。处理所有数据将提供对参与听觉系统独特专业的分子机制的重要新见解。

项目成果

L-type calcium channels in the auditory system

听觉系统中的 L 型钙通道

• DOI: 10.1007/s13295-014-0059-3
• 发表时间: 2014
• 期刊: e-Neuroforum
• 作者: Nothwang HG;Engel J;Knipper M;Friauf E
• 通讯作者: Friauf E