CAREER: Optogenetic Analysis of Neurovascular Coupling

职业：神经血管耦合的光遗传学分析

• 批准号: 1454300
• 负责人: Ramin Pashaie
• 金额: $ 50.65万
• 依托单位: University of Wisconsin-Milwaukee
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2021-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1454300&HistoricalAwards=false
• 关键词: CAREER; Optogenetic; Analysis; Neurovascular; Coupling

PI: Pashaie, RaminProposal: 1454300 This proposal aims at establishing a novel paradigm for astrocytes, which are classically regarded as non-neuronal cells, forming only a support network for neurons. However, recent studies have provided evidence that astrocytes are involved in data processing in the cortex by regulating the neural response magnitude and duration. Furthermore, they appear to have an intermediary role in neurovascular coupling and the signaling pathways that mediate functional hyperemia are regulated by astrocytes. The PI proposes to establish a new platform technology to selectively manipulate neuronal/astrocytic activity and simultaneously record cellular/hemodynamic signals.The PI has established track records in mentoring students and participating in outreach programs. The opportunities for training and teaching of undergraduate and graduate as well as making impact on undergraduate summer research program and K-12 outreach programs are excellent at the University of Wisconsin-Milwaukee. The applicant has provided detailed plans for the development teaching materials and courses that he plans to develop as well as how he plans to mentor and train undergraduate and graduate students. The proposed outreach program is also well developed. The applicant provides clear plan for how he plans to evaluate each component of the proposed teaching and outreach program.The PI will combine optogenetics for modulation of cellular activity of neurons and astrocytes with spectral-domain coherence tomography to monitor hemodynamic signals, and two-photon microscopy to observe cellular activity. Since both cellular activity and hemodynamics will be triggered by means of optogenetic modulation, their correlation permits conclusions about the roles of both, astrocytes and neurons. The optogenetic and microscopy experiments will be complemented by a novel electrophysiology platform that the PI has recently developed for optogenetic electrocorticography (ECoG) experiments. Electrophysiological recording of calcium waves will permit a comparison of astrocyte activity and neuronal activity.This award is co-funded by the Biophotonics program in the Engineering Directorate and the Neural Systems program in the BIO Directorate.

PI：Pashaie，Raminproposal：1454300该提案旨在建立一个新颖的星形胶质细胞范式，而星形胶质细胞通常被视为非神经元细胞，仅形成神经元的支持网络。但是，最近的研究提供了证据，表明星形胶质细胞通过调节神经反应的幅度和持续时间参与了皮质中的数据处理。此外，它们似乎在神经血管耦合中具有中间作用，并且由星形胶质细胞调节介导功能性高血症的信号传导途径。 PI建议建立一种新的平台技术，以选择性地操纵神经/星形胶质细胞活动，并同时记录细胞/血液动力学信号。PI已在指导学生和参加外展计划方面建立了记录记录。威斯康星大学 - 米尔沃基大学（University of Wisconsin-Milwaukee）非常出色，对本科生和毕业生的培训和教学机会以及对本科夏季研究计划和K-12外展计划的影响非常出色。申请人为他计划开发的发展教材和课程提供了详细的计划，以及他计划如何指导和培训本科生和研究生。拟议的外展计划也发达了。申请人为他计划如何评估拟议的教学和外展计划的每个组成部分提供了明确的计划。PI将结合光遗传学，以调节神经元和星形胶质细胞的细胞活性与光谱域相干断层扫描，以监测血液动力学信号，以及两光子的显微镜，并观察到细胞活性。由于细胞活性和血液动力学都将通过光遗传学调节触发，因此它们的相关性允许就星形胶质细胞和神经元的作用得出结论。光学遗传学和显微镜实验将由一个新型的电生理平台进行补充，PI最近为光遗传学电视学（ECOG）实验开发了该平台。钙波的电生理记录将允许对星形胶质细胞活动和神经元活动进行比较。该奖项由生物指导计划在工程局和生物局的神经系统计划中共同资助。