Characterization of the interaction of Get3 with the membrane bound receptors Get1 and Get2

Get3 与膜结合受体 Get1 和 Get2 相互作用的表征

• 批准号: 217561225
• 负责人: Professor Dr. Volker Dötsch
• 金额: --
• 依托单位: Department of Molecular and Cellular Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/217561225?language=en
• 关键词: Characterization; interaction; Get3; membrane; bound

The insertion of membrane proteins into the correct membrane of a cell is an important cellular task. A special class of membrane proteins, called tail-anchored proteins, consist of an N-terminal soluble domain and a single C-terminal transmembrane helix. Insertion of these tail-anchored proteins into membranes requires a specialized machinery that includes several soluble and membrane-bound components. After synthesis at the ribosome the transmembrane helix of the tail-anchored protein is loaded into a hydrophobic groove on the protein Get3. This protein interacts in an ATP-dependent manner with the two membrane bound receptors Get1 and Get2. While Get2 is important for recruiting Get3 to the membrane, the interaction with Get1 opens the closed conformation of Get3 and initiates the insertion process. Recently we have solved the structures of Get3 in complex with the soluble domains of Get1 and Get2. Based on these results we now propose to investigate the structure and function of the entire complex including the transmembrane parts. Both receptors consist of three transmembrane helices each and were shown to form a tight complex. We will use mutagenesis analysis and insertion assays to further characterize this crucial process.

将膜蛋白插入正确的细胞膜是一项重要的细胞任务。一类特殊的膜蛋白，称为尾锚定蛋白，由 N 端可溶结构域和单个 C 端跨膜螺旋组成。将这些尾部锚定蛋白插入膜需要一种专门的机器，其中包括几种可溶性和膜结合成分。在核糖体合成后，尾部锚定蛋白的跨膜螺旋被加载到蛋白 Get3 上的疏水沟中。该蛋白以 ATP 依赖性方式与两个膜结合受体 Get1 和 Get2 相互作用。虽然 Get2 对于将 Get3 招募到膜上很重要，但与 Get1 的相互作用打开了 Get3 的闭合构象并启动插入过程。最近我们解决了Get3与Get1和Get2的可溶结构域的复合体结构。基于这些结果，我们现在建议研究整个复合物（包括跨膜部分）的结构和功能。两种受体均由三个跨膜螺旋组成，并显示出形成紧密的复合物。我们将使用诱变分析和插入测定来进一步表征这一关键过程。