Leveraging Pathogen Diversity for Gaining Insights into Molecular Plant - Microbe Interactions

利用病原体多样性深入了解分子植物-微生物相互作用

• 批准号: 1354215
• 负责人: Boris Vinatzer
• 金额: $ 59.99万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1354215&HistoricalAwards=false
• 关键词: Leveraging; Pathogen; Diversity; Gaining; Insights

Plants are exposed to many pathogens and consequently evolved a sophisticated immune system. On the other hand, pathogens evolved sophisticated mechanisms to overcome the plant immune system. In this project, the investigators will take advantage of genetic diversity in pathogen populations and in crop plants to identify pathogen genes and plant genes that determine the outcome of plant-pathogen interactions. These genes can be expected to provide new avenues towards novel approaches in crop disease prevention and control. The project will also offer multiple opportunities for training students in an interdisciplinary and international context.The investigators previously gained new insights into molecular plant-microbe interactions by comparing strains of the tomato pathogen Pseudomonas syringae pv. tomato (Pto) with each other. In particular, they identified the pathogen-associated molecular pattern (PAMP) flgII, which is an epitope within the flagellar filament structural protein (FliC) and which triggers an immune response in tomato and other Solanaceae. To identify additional PAMPs, an international strain collection of Pto and of its close relatives will be expanded in the current project by isolating bacteria from rain, surface water, and crops. Genome sequencing and population genomic analyses will be performed. Pathogen emergence, spread, and crop adaptation will be investigated and new PAMPs and other genes involved in pathogen - plant interaction will be predicted based on signatures of natural selection. Candidate genes will then be tested for their role in pathogen - plant interaction using biochemical and genetic approaches. Such approaches will also be used to identify the tomato receptor of the previously characterized PAMP flgII and, possibly, tomato receptors of other PAMPs that may be identified in the current project.

植物暴露于许多病原体，因此进化出了复杂的免疫系统。另一方面，病原体进化了复杂的机制来克服植物免疫系统。在该项目中，研究人员将利用病原体种群和作物植物中的遗传多样性来识别确定植物病原体相互作用结果的病原体基因和植物基因。可以预期，这些基因将为作物疾病预防和控制的新方法提供新的途径。该项目还将为在跨学科和国际环境中培训学生提供多个机会。研究人员以前通过比较番茄病原体假单胞菌丁香PV的菌株，获得了对分子植物 - 微生物相互作用的新见解。番茄（PTO）彼此。特别是，他们确定了与病原体相关的分子模式（PAMP）FLGII，它是鞭毛丝结构蛋白（FLIC）中的表位，并触发番茄和其他茄科中的免疫反应。为了识别其他大型助长，通过将细菌与雨水，地表水和农作物隔离来，将在当前项目中扩展国际PTO及其近亲的菌株集合。将进行基因组测序和种群基因组分析。将研究病原体的出现，扩散和作物适应，并根据自然选择的签名预测病原体的新症状和其他基因 - 植物相互作用。然后，将使用生化和遗传方法对候选基因进行病原体 - 植物相互作用的作用进行测试。这种方法还将用于鉴定先前表征的PAMP FLGII的番茄受体，以及可能在当前项目中可以鉴定出的其他PAMP的番茄受体。