FSML: Enabling the next generation of cell analysis at Horn Point Laboratory

FSML：在 Horn Point 实验室实现下一代细胞分析

• 批准号: 1318455
• 负责人: Alyson Santoro
• 金额: $ 26.86万
• 依托单位: University of Maryland Center for Environmental Sciences
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1318455&HistoricalAwards=false
• 关键词: FSML; Enabling; next; generation; cell

The University of Maryland Center for Environmental Sciences is awarded a grant to establish a cell imaging and analysis center at the Horn Point Laboratory (http://www.umces.edu/hpl) to support research and training on the ecology and physiology of phytoplankton and bacterioplankton. Visualizing and counting microorganisms are fundamental to microbial ecology research and discovery. HPL will update and expand its capacity to count and visualize microbial cells to support and enhance existing molecular biology and microbial ecology facilities. Funds will be used to acquire a benchtop high-throughput flow cytometer, an inverted light microscope, and an epifluorescent microscope with a modern imaging system. This equipment will build the capacity for high-throughput cultivation experiments and single-cell substrate uptake analysis using microautoradiography and allow HPL to offer an intensive winter-term course on phytoplankton physiology for graduate and advanced undergraduate students from around the world.Beautiful images of cells are some of the most important tools microbial ecologists have to communicate and disseminate their research and engage the general public. The cell analysis center will become an integral part of graduate education at HPL, where it will be available to the entire Chesapeake Bay and Mid-Atlantic Bight research community. Summer undergraduate students will use the equipment as part of their research projects, as will students enrolled in a winter term short course on phytoplankton physiology and ecology, allowing HPL to train the next generation of microbial ecologists in phytoplankton physiology and identification. Cell staining and counting protocols will be made available via a newly constructed website for the cell analysis center, ensuring broad dissemination of our findings.

马里兰大学环境科学中心获得拨款，在 Horn Point 实验室 (http://www.umces.edu/hpl) 建立细胞成像和分析中心，以支持浮游植物生态学和生理学的研究和培训和浮游细菌。 微生物的可视化和计数是微生物生态学研究和发现的基础。 HPL 将更新和扩展其微生物细胞计数和可视化能力，以支持和增强现有的分子生物学和微生物生态设施。 资金将用于购买一台台式高通量流式细胞仪、一台倒置光学显微镜和一台带有现代成像系统的落射荧光显微镜。该设备将建立使用显微放射自显影技术进行高通量培养实验和单细胞底物摄取分析的能力，并使 HPL 能够为来自世界各地的研究生和高年级本科生提供有关浮游植物生理学的强化冬季学期课程。美丽的细胞图像是微生物生态学家交流和传播他们的研究并吸引公众参与的一些最重要的工具。 该细胞分析中心将成为 HPL 研究生教育不可或缺的一部分，整个切萨皮克湾和中大西洋湾研究界都可以使用该中心。 暑期本科生将使用这些设备作为其研究项目的一部分，参加冬季学期浮游植物生理学和生态学短期课程的学生也将使用这些设备，从而使 HPL 能够培训下一代浮游植物生理学和识别方面的微生物生态学家。 细胞染色和计数方案将通过细胞分析中心新建的网站提供，确保我们的研究结果得到广泛传播。