Collaborative Research: Transport and Separation through Virus-Structured Nanoporous Membranes

合作研究：通过病毒结构纳米多孔膜进行运输和分离

• 批准号: 1264958
• 负责人: Matthew McCarthy
• 金额: $ 24.03万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-15 至 2017-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1264958&HistoricalAwards=false
• 关键词: Collaborative; Research; Transport; Separation; through

McCarthy/Maroo 1264949 / 1264958The objective of the proposed collaborative research is to investigate transport and separation phenomena through the protein channel of the tobacco mosaic virus (TMV) using an integrated research methodology combining molecular analysis and simulations along with direct experimental characterization. The TMV is a rigid, hollow, rod-shaped plant virus with a 4-nm diameter central pore defined by 2130 helical coat proteins wrapped around a single strand of RNA. It is an extremely stable bio-molecule, withstanding temperatures of up to 60 degrees C and a pH range of 2 to 11. The surface of the central pore is negatively charged, making it attractive for ion exclusion. The outer surface of the TMV has been genetically modified to facilitate near-vertical assembly and metallization onto various materials. This feature provides a mechanism to develop virus-structured membranes using large-scale industrially relevant manufacturing schemes. Due to its stability, structure, surface charge, and manufacturability, the TMV can potentially transform membrane manufacture for biological and chemical separations. This collaborative research project will bring together the expertise of one PI (Maroo) in molecular dynamics simulations and numerical modeling with a second PI (McCarthy) in TMV biotemplating and nanoscale fabrication. The project will focus on the following numerical and experimental investigations: (1) Determination of the surfaces properties of the TMV central pore, (2) Numerical and molecular modeling of overlapping electric double layers, (3) Molecular dynamics simulations of transport and ionic exclusion through the TMV central pore, (4) Fabrication of virus-structured nanoporous membranes using the self-assembly of the TMV, (5) Experimental characterization of transport phenomena through the TMV membranes, and (6) Experimental characterization of separation through the TMV including size and ionic exclusion. The synergy of these two components (numerical and experimental) will result in a comprehensive understanding of transport and separation through the TMV and demonstrate the potential of TMV-structured membranes for water filtration and chemical and biological separations. The advantages of utilizing biological building blocks in nano-engineered systems include low cost, structural versatility, inherent self-assembly properties, and the ability to tune structure through genetic modifications and environmental control. The knowledge base gained in this work will act as a catalyst for future development in the field of separations and the nanomanufacturing of bio-derived membranes. Broader Impacts. This work will build on the PI's existing participation in Drexel's NSF-funded GK-12 program on the NAE's Grand Challenges (focusing on water desalination) and Syracuse University's Project Engage where the PI holds workshops on modern engineering solutions for K-12 female students. Outreach will extend to pre-college students, particularly those from underrepresented groups in the Philadelphia metropolitan and Syracuse areas and will focus on exposing undergraduates, women, and minorities to multidisciplinary research through integrated research-education initiatives. Undergraduate students at both Drexel and Syracuse will be recruited for research opportunities and participation in the Workshops on Nanoscale Transport through Protein Channels developed by the PIs in the proposed work.

McCarthy / Maroo 1264949 /1264958所提出的合作研究的目的是通过综合研究方法结合分子分析和模拟以及直接的实验表征，通过烟草马赛克病毒（TMV）的蛋白质通道研究运输和分离现象。 TMV是一种刚性的，空心的棒状植物病毒，其直径为4 nm的中央孔，该孔由2130螺旋涂层蛋白包裹在一条RNA周围。 它是一种极为稳定的生物分子，耐受的温度高达60摄氏度，pH值为2至11。中央孔的表面充满电，使其对离子排除有吸引力。 TMV的外表面已经过遗传修饰，以促进近垂直组装并金属化到各种材料上。 此功能提供了一种使用大规模工业相关的制造方案来开发病毒结构膜的机制。 由于其稳定性，结构，表面电荷和制造性，TMV可以潜在地改变膜制造以进行生物学和化学分离。 该协作研究项目将在分子动力学模拟和数值建模中使用第二个PI（McCarthy）（McCarthy）在TMV Biotemplating和纳米级制造中汇集一项PI（MAROO）的专业知识。 The project will focus on the following numerical and experimental investigations: (1) Determination of the surfaces properties of the TMV central pore, (2) Numerical and molecular modeling of overlapping electric double layers, (3) Molecular dynamics simulations of transport and ionic exclusion through the TMV central pore, (4) Fabrication of virus-structured nanoporous membranes using the self-assembly of the TMV, （5）通过TMV膜对转运现象的实验表征，以及（6）通过TMV分离的实验表征，包括大小和离子排除。 这两个组成部分的协同作用（数值和实验性）将使通过TMV进行对运输和分离的全面了解，并证明TMV结构化膜在水过滤以及化学和生物学分离中的潜力。 在纳米工程系统中利用生物构建块的优势包括低成本，结构多功能性，固有的自组装特性以及通过遗传修饰和环境控制来调整结构的能力。 这项工作中获得的知识基础将充当分离领域未来发展和生物衍生膜制造的纳米制造的催化剂。 更广泛的影响。 这项工作将基于PI的现有参与Drexel的NSF资助的GK-12计划，该计划在NAE的巨大挑战（重点关注水脱水平）和Syracuse University的Project Groment参与其中，PI在这里为K-12女学生提供了现代工程解决方案的研讨会。 外展活动将扩展到大学前的学生，特别是费城都会和锡拉丘兹地区的代表性不足群体的学生，并将专注于通过综合研究教育计划将本科生，妇女和少数群体与多学科研究暴露于多学科研究。 Drexel和Syracuse的本科生将通过PIS在拟议的工作中开发的蛋白质渠道招募研究机会，并通过PIS开发的蛋白质渠道参加纳米级运输的研讨会。