MRI: Acquisition of an Isotope Ratio Mass Spectrometer for Compound-Specific Applications in Biogeochemistry and Environmental Studies at UC Santa Cruz

MRI：购买同位素比质谱仪，用于加州大学圣克鲁斯分校生物地球化学和环境研究中的化合物特定应用

• 批准号: 1828774
• 负责人: Matthew McCarthy
• 金额: $ 56.42万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828774&HistoricalAwards=false
• 关键词: MRI; Acquisition; Isotope; Ratio; Mass

Stable isotope analysis is now one of the most important tools in a remarkable variety of research, with key applications spanning oceanography, biogeochemical cycle research, ecology and food webs, paleoecology, archaeology, and paleoclimatogy. Work in all these areas increasingly requires sensitive analysis of the isotope ratios of a range of elements (carbon, hydrogen, oxygen, nitrogen, sulfur), in a wide variety of sample types, and the most innovative projects increasingly require new, leading-edge approaches, such as isotopic analysis of individual organic compounds (CSIA). This award to the University of California at Santa Cruz (UCSC) involves the acquisition of an isotope-ratio-monitoring mass spectrometer (IRMS) to serve investigators and students across multiple disciplines. This acquisition will have wide broader impacts. First, the instrumentation will significantly expand UCSC's basic research infrastructure, supporting core campus priority areas for excellence in coastal sciences and environmental sustainability research. It strongly enhances UCSC's core education and teaching mission, allowing graduate and post-doc training on cutting-edge instrumentation while supporting a far greater range of projects. The instrumentation will support undergraduate research, via senior theses and direct involvement in research labs, as well as the incorporation of hands-on projects into higher level courses. The project team will also develop a new initiative for a summer outreach program, in collaboration with UCSC's division of student success.UCSC will acquire a Thermo Scientific 253+ isotope ratio mass spectrometer (IRMS), with two front-end peripherals configured for cutting-edge CSIA capabilities, including the ability to make isotopic measurements on unknown compounds, and the addition of sulfur isotope capability. This high-sensitivity IRMS will have two main sample input peripherals: (1) a GC-Trace Ultra-GC coupled to a TSQ Duo Triple Quadrapole Mass Spectrometer, and configured with a programmable Tri-Plus RSH auto-sampler capable of automated derivatization/standard additions; and (2) an IsoLink CNOHS Elemental Analyzer (EA) with a new unified combustion/pyrolysis design that will bring needed sulfur isotope capability. Each component in this configuration was chosen to fulfill a critical need. The 10 KV 253 IRMS will provide high sensitivity, allowing a range of new, nano-scale compound-specific applications, particularly critical for individual-compound N measurements. The customized Tri-Plus RHS auto-sampler can both make and inject derivatives with automated addition of internal standards. This will provide high sample throughput. The TSQ Duo Triple Quadrapole Mass Spectrometer represents a completely novel, cutting-edge capability, allowing researchers to simultaneously identify unknown structures as they measure their C and N isotope ratios. Finally, the EA IsoLink (CNSOH) interface will bring the ability to measure S isotopes to UCSC for the first time, allowing 13C, 15N, and 34S values to be measured simultaneously on a single sample, even at extremely high C/S ratios (e.g., wood samples).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

稳定同位素分析现已成为各种研究中最重要的工具之一，其关键应用涵盖海洋学、生物地球化学循环研究、生态学和食物网、古生态学、考古学和古气候学。所有这些领域的工作越来越需要对各种样品类型中的一系列元素（碳、氢、氧、氮、硫）的同位素比率进行灵敏分析，并且最具创新性的项目越来越需要新的、前沿的方法，例如单个有机化合物的同位素分析 (CSIA)。加州大学圣克鲁斯分校 (UCSC) 获得的该奖项涉及购买同位素比监测质谱仪 (IRMS)，为多个学科的研究人员和学生提供服务。此次收购将产生更广泛的影响。首先，该仪器将显着扩展 UCSC 的基础研究基础设施，支持核心校园优先领域，以实现沿海科学和环境可持续性研究的卓越发展。 它有力地增强了 UCSC 的核心教育和教学使命，允许对尖端仪器进行研究生和博士后培训，同时支持更广泛的项目。 该仪器将通过高级论文和直接参与研究实验室以及将实践项目纳入更高级别的课程来支持本科生研究。该项目团队还将与 UCSC 的学生成功部门合作，制定一项夏季外展计划的新举措。UCSC 将购买一台 Thermo Scientific 253+ 同位素比质谱仪 (IRMS)，配有两个前端外围设备，用于切割-边缘 CSIA 功能，包括对未知化合物进行同位素测量的能力，以及添加硫同位素功能。该高灵敏度 IRMS 将具有两个主要样品输入外围设备：(1) GC-Trace Ultra-GC，与 TSQ Duo 三重四极杆质谱仪耦合，并配置有可编程 Tri-Plus RSH 自动进样器，能够自动衍生/标准添加； (2) IsoLink CNOHS 元素分析仪 (EA)，采用新的统一燃烧/热解设计，可带来所需的硫同位素能力。该配置中的每个组件都是为了满足关键需求而选择的。 10 KV 253 IRMS 将提供高灵敏度，支持一系列新的纳米级化合物特定应用，对于单个化合物 N 测量尤其重要。定制的 Tri-Plus RHS 自动进样器可以通过自动添加内标来制备和注射衍生物。这将提供高样品通量。 TSQ Duo 三重四极杆质谱仪代表了一种全新的尖端功能，使研究人员能够在测量未知结构的 C 和 N 同位素比率时同时识别它们。 最后，EA IsoLink (CNSOH) 接口将首次为 UCSC 带来测量 S 同位素的能力，即使在极高的 C/S 比下，也可以在单个样品上同时测量 13C、15N 和 34S 值（该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。