Collaborative Research: Exploiting synthetic GPCRs and mating factors as extracellular sensors for substrate-dependent assembly of complex cellulosomes

合作研究：利用合成 GPCR 和交配因子作为细胞外传感器，用于复杂多纤维素酶体的底物依赖性组装

基本信息

批准号：
1263799
负责人：
Nancy Da Silva
金额：
$ 18万
依托单位：
University of California-Irvine
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2017-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1263799&HistoricalAwards=false
关键词：
Collaborative Research Exploiting synthetic GPCRs

项目摘要

1263719/1263768/1263799Chen/Robinson/Da Silva The primary obstacle impeding the more widespread use of biomass for energy and chemical production is the absence of a low-cost technology for overcoming the recalcitrance of these materials. Cellulosomes are self-assembled multi-enzyme complexes found in many anaerobic microorganisms that are highly efficient for biomass depolymerization. The group of investigators have successfully developed a synthetic yeast consortium displaying a functional cellulosome for biofuel production. However, given the complexity of the different members involved in the consortium, engineering cell to cell coordination is the key in maintaining the required overall functionality. Their goal in this project is to design synthetic extracellular sensors that can be used to regulate the secretion of enzymes and adaptor scaffoldins involved in the assembly of the appropriate complex cellulosome structure in a substrate-dependent manner. The proposed research is scientifically significant because it will provide a general framework for the design of extracellular sensors that are important for other bioprocessing or biomedical applications. Moreover, graduate students participating in this research will gain an integrated perspective of the important interfaces and synergies connecting biochemistry, modern genetics, and process engineering to solve an important problem of our society.

1263719/1263768/1263799Chen/Robinson/Da Silva 阻碍生物质更广泛地用于能源和化学品生产的主要障碍是缺乏克服这些材料的顽固性的低成本技术。纤维素体是在许多厌氧微生物中发现的自组装多酶复合物，对生物质解聚非常有效。研究小组成功开发了一种合成酵母联盟，展示了用于生物燃料生产的功能性纤维素体。然而，考虑到联盟中不同成员的复杂性，工程单元之间的协调是维持所需整体功能的关键。他们在该项目中的目标是设计合成的细胞外传感器，可用于调节酶和接头支架蛋白的分泌，这些酶和接头支架蛋白以底物依赖性方式参与适当的复杂纤维素体结构的组装。拟议的研究具有科学意义，因为它将为细胞外传感器的设计提供一个通用框架，这对于其他生物加工或生物医学应用很重要。此外，参与这项研究的研究生将获得连接生物化学、现代遗传学和过程工程的重要界面和协同作用的综合视角，以解决我们社会的重要问题。