GOALI: Metabolic Engineering of Yeast for Polyketide Synthesis

目标：用于聚酮化合物合成的酵母代谢工程

• 批准号: 0432307
• 负责人: Nancy Da Silva
• 金额: $ 11.77万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0432307&HistoricalAwards=false
• 关键词: GOALI; Metabolic; Engineering; Yeast; Polyketide

DaSilva0432307Polyketides are a diverse group of natural products with great significance as human therapeutics. Although they are made in a variety of microorganisms, polyketides often are produced in their natural hosts in minute quantities. To optimize production of these compounds and for the generation of novel polyketides, heterologous hosts hold several advantages. The specific attributes of yeast combined with success at the high-level production of a fungal polyketide in Saccharomyces cerevisiae point to the exceptional promise of this host for polyketide biosynthesis. The Principal Investigator (PI) will develop and evaluate various strategies for introducing and expressing active polyketide synthases in S. cerevisiae.

DaSilva0432307聚酮化合物是一组多样化的天然产物，对于人类治疗具有重要意义。 尽管它们是由多种微生物产生的，但聚酮化合物通常在其天然宿主中产生微量。为了优化这些化合物的生产并生成新型聚酮化合物，异源主体具有多种优势。酵母的特殊属性加上在酿酒酵母中高水平生产真菌聚酮化合物的成功，表明该宿主在聚酮化合物生物合成方面具有非凡的前景。首席研究员 (PI) 将开发和评估在酿酒酵母中引入和表达活性聚酮合酶的各种策略。