Collaborative Research: AToL: ACCESS DNA viruses: A Comprehensive survey of Circular Eukaryotic Single-Stranded DNA viruses in Invertebrates and Fungi

合作研究：AToL：ACCESS DNA 病毒：无脊椎动物和真菌中环状真核单链 DNA 病毒的综合调查

• 批准号: 1239976
• 负责人: Mya Breitbart
• 金额: $ 99.54万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-01 至 2018-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1239976&HistoricalAwards=false
• 关键词: Collaborative; Research; AToL; ACCESS; DNA

Viruses affect all forms of cellular life; however, it is extremely difficult to trace their evolutionary history. This project will reconstruct the phylogeny of an understudied branch of the viral tree of life: that of circular eukaryotic single-stranded DNA (CESS) viruses. CESS viruses are the smallest known viruses, yet they include pathogens with devastating impacts on agriculture worldwide. Recently, general environmental sampling has uncovered a diversity of CESS viruses that are only distantly related to these pathogens and has shown that they infect a wider range of hosts than previously thought, including insects and fungi. This project systematically tests previously unexplored hosts to discover, sequence, and classify their CESS viruses. Phylogenetic analyses will then determine how all CESS viruses are related to one another, providing a framework for classifying the CESS viral tree of life.Broader impacts of this research include training of graduate students, undergraduates, and postdocs, as well as K-12 outreach and creation of web-based resources for the scientific and general public. Middle school students will be involved in collecting invertebrate samples, which will be processed during a virology training workshop for graduate students and postdocs on CESS virus genomics. Project results will be displayed on a website to increase public knowledge about CESS viruses, and bioinformatics tools will be shared freely with the scientific community.

病毒影响所有形式的细胞生命；然而，追踪它们的进化历史却极其困难。该项目将重建病毒生命树的一个尚未研究的分支的系统发育：环状真核单链 DNA (CESS) 病毒的系统发育。 CESS 病毒是已知最小的病毒，但它们包含对全球农业造成毁灭性影响的病原体。最近，一般环境采样发现了多种 CESS 病毒，这些病毒与这些病原体只有远​​亲关系，并表明它们感染的宿主范围比以前想象的更广泛，包括昆虫和真菌。该项目系统地测试以前未探索过的宿主，以发现、排序和分类其 CESS 病毒。然后，系统发育分析将确定所有 CESS 病毒如何相互关联，从而提供对 CESS 病毒生命树进行分类的框架。这项研究的更广泛影响包括对研究生、本科生和博士后的培训，以及 K-12 的推广为科学界和公众创建网络资源。中学生将参与收集无脊椎动物样本，这些样本将在 CESS 病毒基因组学研究生和博士后病毒学培训研讨会上进行处理。项目结果将在网站上展示，以增加公众对 CESS 病毒的了解，生物信息学工具将与科学界免费共享。