Implementation of continuous flow cytometry for high resolution mapping of microbial distributions in surface waters

实施连续流式细胞术以高分辨率绘制地表水中微生物分布图

• 批准号: 1154074
• 负责人: Virginia Armbrust
• 金额: $ 87.39万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1154074&HistoricalAwards=false
• 关键词: Implementation; continuous; flow; cytometry; resolution

The PI's request funding to complete the development of instrumentation that allows continuous high-resolution mapping of microbial (both autotrophic and heterotrophic) communities in surface waters. With this proposal they propose to transition SeaFlow into the final development stage where it will be permanently installed and operated on the R/V Thompson, requiring minimal support of shipboard marine technicians. This instrument will build upon SeaFlow optics and fluidics and will be the first instrument to continuously measure the distribution of heterotrophic microbes in surface waters. They will also develop a collaborative data processing, management and analysis platform to allow scalable, interactive analysis of our rapidly expanding data sets both on-vessel and on-shore, during and after the cruise. Broader Impacts: A successful outcome from this proposal could pioneer the routine use of flow cytometry on ships of opportunity and on research cruises where a specialist in this technique was not present. The increased frequency and coverage of sampling, and the proposed ease of access to the data, is likely to stimulate interest in marine microbial ecology in the wider oceanographic community. The proposed research will provide great training opportunities for undergraduates and post doctoral researchers directly and will presumably include graduate students in some of the real time testing and feedback. This project is definitely interdisciplinary and pulls in undergraduates from different disciplines.

PI的请求资金以完成仪器的发展，该仪器允许对地表水域中微生物（自养和异养）群落进行连续的高分辨率映射。通过这项建议，他们建议将海流转变为最终开发阶段，在R/V Thompson将永久安装和操作它，需要最小的支持船舶海洋技术人员的支持。 该仪器将建立在海流光学和流体上，并将成为第一个连续测量地表水中异养微生物分布的仪器。 他们还将开发一个协作数据处理，管理和分析平台，以允许对我们在巡航期间和之后的官方和近岸迅速扩展的数据集进行可扩展的交互式分析。更广泛的影响：该提案的成功结果可能会在机会船上的流式细胞仪和研究巡游中常规使用，而该技术不存在该技术的专家。采样的频率和覆盖范围的增加以及拟议的易于获取数据的频率可能会刺激更广泛的海洋学社区对海洋微生物生态学的兴趣。拟议的研究将为本科生和博士后研究人员提供很大的培训机会，并且大概将在一些实时测试和反馈中包括研究生。该项目绝对是跨学科的，并从不同学科中吸引了本科生。