Collaborative Research: IMAGiNE: Quantifying Diatom Resilience in an Acidified Ocean

合作研究：IMAGiNE：量化酸化海洋中硅藻的恢复力

• 批准号: 2051212
• 负责人: Virginia Armbrust
• 金额: $ 39.3万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2051212&HistoricalAwards=false
• 关键词: Collaborative; Research; IMAGiNE; Quantifying; Diatom

This research seeks to investigate how long-term environmental changes like ocean acidification will affect diatoms, a key microscopic phytoplankton forming the basis of many marine food webs. Diatoms account for ~40 percent of the primary production in our oceans, and a shift in their composition and abundance may result in dramatic changes in coastal ecosystems. A stress test will be developed to quantify the “resilience” of diatoms, i.e., the degree to which they can withstand environmental stress such as saturating light, ultraviolet radiation, or increased temperature. By applying this stress test to three model diatoms that inhabit different oceanic environments, Thalassiosira oceanica, Phaeodactylum tricornutum, and Thalassiosira pseudonana, this study will uncover whether ocean acidification will have similar or distinct consequences on the future fate of these important organisms. Furthermore, this study will also characterize molecular mechanisms responsible for the observed shifts in the resilience of diatoms. Mechanistic understanding of how ocean acidification might alter the resilience of diatoms will enable predictive and actionable strategies for better environmental stewardship. Additionally, this project will generate new high school curriculum on the concepts of resilience and collapse of complex systems encountered in our everyday life. The curricula will be disseminated widely through teacher training.Diatoms have evolved phenotypic plasticity to survive in fluctuating environments, and the capability to tolerate diverse types of stress. The proposed research addresses the challenge of quantifying how diatoms manage trade-offs between maintaining phenotypic plasticity and devoting resources to mitigating stress, which is central to predicting their resilience in complex environments. The stress test framework will enable the quantification of ecological resilience of a diatom, i.e., the degree to which a diatom population can tolerate a disturbance and persist without changing physiological state. By performing the stress test on three model diatoms representing different ecological niches, and in relevant conditions of current and future oceans (i.e., temperature, CO2, NO3, Fe, and light conditions), this study will allow the prediction of when interactions among specific factors will have synergistic or antagonistic effects on the resilience of diatoms. Systems level analysis of transcriptional (RNA-seq) and physiological changes coupled to hypothesis testing using CRISPR-cas9-based genome editing will provide predictive and mechanistic understanding of changes in diatom resilience in dynamic environments. The resulting knowledge, framework, and tools will serve as predictive indicators to forecast species partitioning and shifts in ecosystem function in changing oceans. Furthermore, the stress test framework and systems approaches will be generalizable to investigate resilience and other complex traits across microbial communities of environmental importance. This award is cofunded by the Division of Integrative Organismal Systems and the Division of Molecular and Cellular Biosciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项研究旨在调查海洋酸性硅藻等海洋酸性硅藻的变化，构成和丰度的基础可能会导致沿海生态系统的巨大变化。例如饱和光，紫外线辐射，或通过三个模型的海洋环境（Thalassiosira Oceanica，Tricornutum，andylassiosira pseudonana）施加了测试。硅化的韧性变化可能会改变硅硅藻的韧性，该项目带有新的高中curraliculum，在我们的训练中遇到的复杂系统崩溃的概念在波动中生存的塑料以及耐受类型的能力。硅藻，即具有生理状态的干扰和持续的程度。 ，这项研究将预测特定因素之间的相互作用将对硅藻的弹性具有协同或拮抗作用。以及对知识变化，框架和工具的变化的机理理解，将作为预测物种分区的谓词，并在生态系统中转移Ong ceans，此外，应力测试框架和系统将被推广到调查和其他复杂特征环境重要性。综合有机体系统和分子生物群体的划分。此奖项Reflems NSF'SF'SF'sFly使命，并被认为是通过Toundation的智力优点和更广泛影响的审查标准来评估的。