Synthesis and Studies of Water-Soluble, Alpha-Helical Polypeptide with Charged Side Chains

带电荷侧链的水溶性α螺旋多肽的合成与研究

• 批准号: 1153122
• 负责人: Jianjun Cheng
• 金额: $ 27.6万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1153122&HistoricalAwards=false
• 关键词: Synthesis; Studies; Water; Soluble; Alpha

In this project funded by the Macromolecular, Supramolecular and Nanochemistry Program of the Chemistry Division, Jianjun Cheng of the University of Illinois at Urbana-Champaign will synthesize and study water-soluble, alpha-helical polypeptides with charged side chains. Polypeptides are important materials with broad applications and characteristic secondary structures. Water-soluble polypeptides bearing charged side chains typically adopt random coil conformations due to side-chain charge repulsion. In this study, we aim to develop water-soluble, alpha-helical polypeptides by elongating charge-containing amino-acid chains to position the charges distally from the polypeptide backbone and then to study their biophysical properties. Preliminary studies will be performed to test these polymers for use in cell penetration and bacterial inhibition. The broader impacts involve training undergraduate and graduate students, participating in summer high school outreach and undergraduate broadening participation activities at the University of Illinois, and disseminating research results through publications in journals and presentations at conferences. Peptides are chains of chemically linked amino acids and are the components of proteins. This work will expand the repertoire of chemistry that can be used to create new large molecules that mimic natural biomaterials and will lead to a better understanding of how peptides can be engineered to form stable secondary structures, such as an alpha-helix, in water. The results of these studies could have many important long term impacts on applications in which protein-based materials are important, including long term applications in medicine, pharmaceutical processing, and nanotechnology.

在这个由化学部的大分子，超分子和纳米化学计划资助的项目中，伊利诺伊大学Urbana-Champaign的江恩·郑（Jianjun Cheng）将综合并研究水溶性，α-高螺旋型多肽，带有带电的侧链。多肽是具有广泛应用和特征二级结构的重要材料。 带有带电侧链的水溶性多肽通常由于侧链电荷排斥而采用随机线圈构象。 在这项研究中，我们旨在通过拉长含电荷的氨基酸链来开发水溶性的α-螺旋多肽，以将电荷从多肽骨架远端定位，然后研究其生物物理特性。 将进行初步研究，以测试这些聚合物用于细胞渗透和细菌抑制作用。 更广泛的影响涉及培训本科生和研究生，参加夏季高中外展活动以及伊利诺伊大学的大学扩大参与活动，并通过会议上的期刊和演讲中的出版物来传播研究结果。肽是化学连接的氨基酸的链，是蛋白质的成分。 这项工作将扩大化学曲目，可用于创建模仿天然生物材料的新的大分子，并可以更好地理解如何在水中设计肽以形成稳定的二级结构，例如α-螺旋体。 这些研究的结果可能会对基于蛋白质材料很重要的应用产生许多重要的长期影响，包括长期应用在医学中，药物加工和纳米技术。