Multidimensional "omics" characterization of microbial metabolism and dissolved organic matter in Antarctica

南极洲微生物代谢和溶解有机物的多维“组学”表征

• 批准号: 1141978
• 负责人: Christine Foreman
• 金额: $ 27万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1141978&HistoricalAwards=false
• 关键词: Multidimensional; omics; characterization; microbial; metabolism

Uncovering the dynamics of dissolved organic matter (DOM) is central to an understanding of the global carbon cycle, as organic material from lakes, streams, oceans and soils passes through this pool. DOM acts as a key energy source for microbes in many ecosystems and therefore can affect regional nutrient cycling patterns. For example, preliminary results suggest that organisms isolated from a supraglacial stream on Cotton Glacier, Antarctica, may be important in DOM cycling in this relatively simple, low temperature system. However, little is known about the functional attributes of the microbes that interact with DOM in the environment. This project will use state-of-the-art genomics, proteomics and metabolomics approaches to understand the mechanisms by which two microbial isolates, CG3 and CG9_1, affect DOM cycling. Liquid chromatography-mass spectrometry will also be used to better characterize the microbially-derived DOM from this ecosystem. This project will support the research and training of one undergraduate and two graduate students. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. Understanding the relationship between cold-adapted microbial metabolisms and DOM pools is important as more than 90% of the Earth?s oceans are below 5 degrees Celsius.

发现溶解有机物（DOM）的动力学是对全球碳循环的理解至关重要的，因为来自湖泊，溪流，海洋和土壤的有机材料通过该池。 DOM充当许多生态系统中微生物的关键能源，因此会影响区域营养循环模式。例如，初步结果表明，从南极洲棉花冰川上的冰川上流分离出的生物可能在这个相对简单的低温系统中对DOM循环很重要。但是，对于与环境中与DOM相互作用的微生物的功能属性知之甚少。该项目将使用最先进的基因组学，蛋白质组学和代谢组学方法来了解两种微生物分离株CG3和CG9_1影响DOM循环的机制。液相色谱 - 质谱法还将用于更好地表征该生态系统中微生物衍生的DOM。该项目将支持一名本科生和两名研究生的研究和培训。 结果将通过出版物以及在国家和国际会议上的演讲来广泛传播。此外，将通过开放访问数据库提供原始数据。了解冷适应的微生物代谢与DOM池之间的关系很重要，因为超过90％的地球海洋低于5摄氏度。