Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core

WAIS Divide 重复核心中溶解有机碳和微生物多样性的分子水平表征

• 批准号: 1141936
• 负责人: Christine Foreman
• 金额: $ 16.23万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1141936&HistoricalAwards=false
• 关键词: Molecular; Level; Characterization; Dissolved; Organic

This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.

该奖项支持南极冰芯中溶解有机碳和微生物的详细分子水平表征。使用最现代的生物学（基因组），地球化学技术和先进的化学仪器研究人员将1）优化用于从适合在下一代pyrosequern中使用的深层冰核中收集，提取和扩增DNA的方案； 2）确定冰芯内的微生物多样性； 3）通过超高分辨率傅立叶变换离子回旋共振质谱法（FT-ICR-MS）获得并分析冰中碳的详细分子特征。使用此试点研究，研究人员将能够量化执行这些特征所需的材料（微生物生物量和碳）的量，这是为将来的冰芯项目提供信息所需的。最终目标是开发最大化产量的协议，同时最大程度地减少所需的冰量。 更广泛的影响包括地方和国家一级的教育和外展。作为蒙大拿州立大学的美洲印第安人研究机会和桥梁计划的教师导师，工头将在夏季在实验室中担任美国原住民学生的导师。苏珊·凯利（Susan Kelly）是一位教育和外展协调员，拥有地质学的MS学位和10年以上的科学外展经验。 她将协调与哈丁学区全面的教育合作的努力，讨论蒙大拿州中南部的乌拉克印第安人保留地。