MRI: Acquisition of an Applied Biosystems 3500xL DNA Analyzer for Interdisciplinary Genomics Research and Training at the University of Houston-Clear Lake

MRI：休斯敦大学克利尔湖分校购买了 Applied Biosystems 3500xL DNA 分析仪，用于跨学科基因组学研究和培训

• 批准号: 1126547
• 负责人: Larry Rohde
• 金额: $ 20.37万
• 依托单位: University of Houston - Clear Lake
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1126547&HistoricalAwards=false
• 关键词: MRI; Acquisition; Applied; Biosystems; 3500xL

This grant will provide for the acquisition of an Applied Biosystems 3500xL 24-capillary DNA Analyzer to foster research and research training capabilities at the University of Houston-Clear Lake (UHCL). The ABI 3500xL DNA Analyzer is a fully automated capillary electrophoresis system with a capacity of sequencing up to 400,000 base pairs per day. The requested equipment will be used by at least eight faculty members in biology, biotechnology and environmental science for their research activities as well as for research training of undergraduate and graduate students. The DNA Analyzer will be utilized to detect pesticide resistance-associated gene mutations in Rhipicephalus microplus, to study essential amino acid biosynthesis in Medicago truncatula, to study molecular mechanisms of environmental stress in soft corals, to study molecular mechanisms involved in the maintenance of zinc homeostasis in plants, to study microbial populations in Galveston Bay, and to study regulation of human inducible nitric oxide synthase. Acquisition of the high-throughput DNA Analyzer will enhance faculty research and advance student learning by enriching courses in genomics and through integration of hands-on exposure for independent research projects using modern instrumentation. Faculty and student researchers will use the equipment to address important problems in the areas of biology, biotechnology and environmental science. For example, the DNA Analyzer will be used to determine the genes responsible for pesticide resistance in the Texas cattle tick, which has led to major loss to the cattle industry. The Analyzer will be used to address problems related to human welfare, such as the role of nitric oxide in the human body, and how to improve the nutritional quality of plant foods, such as legumes, to address the worldwide problem of malnutrition from plant-based diets. The Analyzer will also be used to determine the effects of environmental stress on soft corals, to characterize the microbial community of Galveston Bay, and in other projects important to the environment. This equipment will greatly enhance the generation of research publications and grant proposals from UHCL and enhance ongoing collaborations with regional and national organizations such as the USDA Children?s Nutrition Research Center, USDA-ARS-Livestock Insect Research Laboratory, Nalco Chemical company, and Baylor College of Medicine. Acquisition of this equipment will address both local and national needs for well-trained B.S. and M.S graduates with research skills in biology, biotechnology and environmental science. At least 50 B.S. and M.S. students per year will use the instrument in independent studies and thesis research and another 30 students in formal training courses. A high percentage of these students will be from groups underrepresented in science. Local 7th to 12th grade science students will also be given opportunities to access data from this equipment to learn how to perform genetic and genomic analysis.

这笔赠款将用于购买 Applied Biosystems 3500xL 24 毛细管 DNA 分析仪，以培养休斯顿大学克利尔湖分校 (UHCL) 的研究和研究培训能力。 ABI 3500xL DNA 分析仪是一款全自动毛细管电泳系统，每天能够测序多达 400,000 个碱基对。所请求的设备将供至少八名生物学、生物技术和环境科学领域的教员用于他们的研究活动以及本科生和研究生的研究培训。 DNA分析仪将用于检测微扇头扇贝农药抗性相关基因突变、研究蒺藜苜蓿必需氨基酸生物合成、研究软珊瑚环境应激的分子机制、研究维持锌稳态的分子机制在植物中，研究加尔维斯顿湾的微生物种群，并研究人类诱导型一氧化氮合酶的调节。 购买高通量 DNA 分析仪将通过丰富基因组学课程以及整合使用现代仪器的独立研究项目的实践经验来加强教师研究并促进学生学习。教师和学生研究人员将使用这些设备来解决生物学、生物技术和环境科学领域的重要问题。例如，DNA分析仪将用于确定德克萨斯牛蜱中负责杀虫剂抗性的基因，这导致了养牛业的重大损失。该分析仪将用于解决与人类福祉相关的问题，例如一氧化氮在人体内的作用，以及如何提高豆类等植物性食品的营养质量，以解决全球范围内植物性营养不良的问题。基于饮食。该分析仪还将用于确定环境压力对软珊瑚的影响，描述加尔维斯顿湾微生物群落的特征，以及其他对环境重要的项目。该设备将极大地提高 UHCL 的研究出版物和资助提案的生成，并加强与美国农业部儿童营养研究中心、美国农业部农业研究局牲畜昆虫研究实验室、纳尔科化学公司和贝勒等地区和国家组织的持续合作医学院。购买该设备将满足当地和国家对训练有素的学士学位的需求。以及具有生物学、生物技术和环境科学研究技能的硕士毕业生。 至少 50 个学士学位和硕士。每年有 30 名学生将在独立学习和论文研究中使用该工具，另外 30 名学生将在正式培训课程中使用该工具。这些学生中很大一部分来自科学领域代表性不足的群体。当地 7 至 12 年级的理科学生也将有机会访问该设备的数据，以学习如何进行遗传和基因组分析。