Selection and Delivery of Substrates to the Essential Clp Protease Complex in Plastids of Arabidopsis Thaliana

拟南芥质体中必需 Clp 蛋白酶复合物的底物选择和递送

• 批准号: 1021963
• 负责人: Klaas van Wijk
• 金额: $ 67.5万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-10-01 至 2014-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1021963&HistoricalAwards=false
• 关键词: Selection; Delivery; Substrates; Essential; Clp

Intellectual Merit:Plastids are essential organelles that are present in every plant cell. Plastids include chloroplasts, the sites of photosynthetic energy generation in plants and algae, amyloplasts, which store starch in roots and tubers, and chromoplasts, which accumulate carotenoids in fruits. Around 14% percent of genes in the plant genome are predicted to encode plastid proteins, underlining the importance of the plastid for plant biology. This project concerns the function and substrates of the essential plastid localized Clp protease machinery in the model plant Arabidopsis. The ClpPR protease is a double ring shaped complex containing 10 different ClpPR proteins, most of which are essential for embryogenesis or seedling development. Attached to the barrel are small ClpS1,2 proteins with unknown function and unique to photosynthetic organisms. Intracellular proteases have many functions and must be highly selective in recognizing their substrates. The plastid-localized Clp proteins are clearly important for plastid biogenesis and differentiation, but their mechanism of action in plastids, including Clp substrates and substrate recognition mechanisms, are unknown. The objectives of the project are to identify Clp protease substrates and their degradation signals, to determine the role of ClpS in substrate delivery to the Clp protease system, to determine the function of ClpT1,T2 as a specific plastid component of the Clp system, and to test potential substrates and degradation signals (degrons) identified in objectives 1, 2 and 3 (or in new literature) in vivo. Broader impacts: This project will help to determine the molecular function and organization of the Clp protease system, as well as its substrates and substrate recognition mechanisms. Understanding the role of the Clp proteolytic machinery and substrate recognition signals is also critical to control protein stability with the objective to improve the plastid as the site for overproduction of foreign proteins with nutritional or other added value. The proposed research will provide multi-disciplinary training in plant molecular genetics and biochemistry at the undergraduate, graduate and post-doctoral levels. The PI will participate in curriculum development workshops for high school teachers to develop genomics-based modules for the classroom.

智力优点：质体是每个植物细胞中存在的必不可少的细胞器。质体包括叶绿体，植物和藻类的光合作用产生的位点，淀粉样品，它们将淀粉存储在根和块茎中，以及染色体成形棒，这些淀粉会在水果中积累类胡萝卜素。预计植物基因组中约有14％的基因编码塑料蛋白，这强调了质体对植物生物学的重要性。该项目涉及模型植物拟南芥中必需质体局部CLP蛋白酶机械的功能和底物。 CLPPR蛋白酶是一种包含10种不同CLPPR蛋白的双环复合物，其中大多数对于胚胎发生或幼苗发育至关重要。附着在枪管上的是小的Clps1,2蛋白质，具有未知功能，光合生物独有。细胞内蛋白酶具有许多功能，并且在识别其底物方面必须具有高度选择性。塑料定位的CLP蛋白对于质体的生物发生和分化显然很重要，但是它们在包括CLP底物和底物识别机制在内的质体中的作用机理是未知的。该项目的目标是识别CLP蛋白酶底物及其降解信号，以确定CLP在底物向CLP蛋白酶系统中的作用，以确定CLPT1，T2作为CLP系统的特定质体组件的功能，并且测试在体内1、2和3（或新文献中）中确定的潜在底物和降解信号（DEGRONS）。更广泛的影响：该项目将有助于确定CLP蛋白酶系统及其底物和底物识别机制的分子功能和组织。了解CLP蛋白水解机械和底物识别信号的作用对于控制蛋白质稳定性的目的也至关重要，以改善质体作为过度生产具有营养或其他附加值的外国蛋白质的位点。拟议的研究将在本科，研究生和博士后水平提供植物分子遗传学和生物化学的多学科培训。 PI将参加课程开发研讨会，供高中教师开发基于基因组学的课堂模块。