Cellulase Discovery Via Chemical Complementation

通过化学互补发现纤维素酶

• 批准号: 0957569
• 负责人: Virginia Cornish
• 金额: $ 54万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0957569&HistoricalAwards=false
• 关键词: Cellulase; Discovery; Via; Chemical; Complementation

This award in the Chemistry of Life Processes (CLP) program supports work by Professor Virginia Cornish at Columbia University to carry out fundamental studies on technology to discover cellulase enzymes for biofuel production. One of the major bottlenecks to cost-competitive conversion of biomass to fermentable sugars for biofuel production is the high cost of the cellulase enzymes needed for biomass degradation. The Cornish laboratory has engineered a general system for coupling the growth of yeast to enzyme activity, a system that has now been adapted to detect cellulase activity and facilitate the identification of new cellulases. This strain is now being optimized so that it can be used to identify active enzymes from very large pools of candidate cellulases and is being used to search the DNA of microorganisms for novel cellulase enzymes. The optimization of the laboratory's strains around the cellulase application will also further the technology more generally, which will ultimately allow it to be applied to the detection of other hydrolase enzymes needed for biofuel production and to other classes of enzymes. This research has the potential to identify improved cellulase enzymes that could make the conversion of biomass to biofuels and other commercial products more cost-efficient, helping to reduce our dependence on fossil fuels for energy.

该奖项在《生命过程》中（CLP）计划中的该奖项支持哥伦比亚大学弗吉尼亚·康沃尔教授的工作，以对技术进行基础研究，以发现纤维素酶生产的纤维素酶。 生物量转化为生物燃料生产的可发酵糖的成本竞争力转化的主要瓶颈之一是生物质降解所需的纤维素酶酶的高成本。 康沃尔实验室已经设计了一个通用系统，用于耦合酵母与酶活性的生长，该系统现已适应以检测纤维素酶活性并促进新纤维素酶的鉴定。 现在正在优化该菌株，以便可以用来从非常大的候选纤维酶中识别活性酶，并用于搜索微生物的DNA以寻找新型纤维素酶。 实验室围绕纤维素酶应用周围的菌株的优化也将更广泛地进一步进一步进一步，最终将其应用于检测生物燃料生产所需的其他水解酶和其他类别的酶。 这项研究有可能确定改进的纤维素酶酶，这可能会使生物量转化为生物燃料和其他商业产品的成本效益，从而减少我们对化石燃料的能源的依赖。