CAREER: An In Vivo Selection for Enzymatic Activity

职业生涯：酶活性的体内选择

• 批准号: 9984928
• 负责人: Virginia Cornish
• 金额: $ 47万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-15 至 2004-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9984928&HistoricalAwards=false
• 关键词: CAREER; Vivo; Selection; Enzymatic; Activity

This project comprises research goals as well as a complementary education plan. The research goals involve the development of high-throughput screening techniques for libraries of proteins. A unique approach is involved that brings together synthetic organic chemistry and molecular genetics, in which bond formation or cleavage can be detected because the bond is used to control a cellular readout that depends on protein dimerization. This general strategy is anticipated to have broad applications ranging from enzymology and enzyme design to functional genomics and drug discovery.With this CAREER Award, the Organic and Macromolecular Chemistry Program's Organic Dynamics Program supports the research of Professor Virginia W. Cornish of Columbia University. Professor Cornish will design and assemble a molecular system to assess the potential effects of small proteins or other small molecules as they affect a given biochemical process. The educational plan has been designed to help students succeed in a research environment that is increasingly interdisciplinary. At the undergraduate level, topic-based teaching modules are being incorporated into the traditional organic chemistry curriculum, and a graduate-level seminar series will address career development issues ranging from balancing work and personal life to giving talks.

该项目包括研究目标以及互补的教育计划。 研究目标涉及开发针对蛋白质库的高通量筛选技术。 一种独特的方法涉及将合成有机化学和分子遗传学融合在一起的独特方法，其中可以检测到键形成或裂解，因为该键用于控制取决于蛋白质二聚体的细胞读数。 预计这种一般策略将具有广泛的应用，从酶学和酶设计到功能性基因组学和药物发现。在该职业奖中，有机和巨型分子化学计划的有机动力学计划支持哥伦比亚大学弗吉尼亚W. Cornish教授的研究。 康沃尔教授将设计和组装一个分子系统，以评估小蛋白或其他小分子的潜在影响，因为它们会影响给定的生化过程。 该教育计划旨在帮助学生在越来越跨学科的研究环境中取得成功。 在本科阶段，基于主题的教学模块正在纳入传统的有机化学课程中，研究生级研讨会系列将解决从平衡工作和个人生活到演讲的职业发展问题。