Genomic Analysis of Unculturable Microbial Eukaryotic Plankton of the San Pedro Ocean Time Series Station

圣佩德罗海洋时间序列站不可培养微生物真核浮游生物的基因组分析

• 批准号: 0732066
• 负责人: Karla Heidelberg
• 金额: $ 50万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0732066&HistoricalAwards=false
• 关键词: Genomic; Analysis; Unculturable; Microbial; Eukaryotic

The availability of genome sequences provides the foundation for understanding how microorganisms function and live, and how they interact with their environments. Ocean microbes are thought to represent one of the greatest untapped sources of biodiversity. However, sub-disciplines studying marine prokaryote microbes, such as bacteria, are much further advanced than those studying more recently evolved unicellular eukaryotic life forms. Large and well-established prokaryote databases are available due to many recent concerted research efforts, while baseline genomic information for marine microscopic eukaryotes is far less advanced. Frequently used molecular methods to evaluate taxonomic diversity provide limited information on potential ecological roles or function of identified organisms. The program described here is designed to obtain large quantities of detailed DNA sequence data linked to known organisms. Data will be obtained for four previously understudied marine taxa from the Kingdoms Euglenozoa, Alveolata, Stramenopila and Cercozoa. These more detailed data will significantly increase genomic information on environmental microbial eukaryotes in the public domain and begin to allow detailed studies of relationships between and among prokaryote and eukaryote taxa. The sampling will take place at the University of Southern California's (USC) well-studied oceanographic time series station in the San Pedro channel, where the research team will have access to abundant biological and chemical data collected by other scientists working at the same site. This study holds tremendous promise to unlock an area of marine microbial research not well studied and will help to advance the state of knowledge within the field of marine microbiology and marine metagenomics.In addition to basic research, the project will also have several significant broader educational impacts. Genomics is a rapidly developing field that is often misunderstood by the general population. The information resulting from the research will enhance teaching curriculum and will provide opportunities for both undergraduate and graduate level research. The research team will partner with USC Wrigley Institute for Environmental Studies (WIES) educators working to develop broad based educational outreach programs for K through grey audiences. The WEIS program is an extraordinary platform for education, and the work is ideally suited to enhance their existing programs. The research team will also participate directly in an ongoing teacher education enhancement program (the NSF funded Centers for Ocean Science Education Excellence; COSEE-West) run by the USC Wrigley Institute, UCLA and several informal science partners. The COSEE-West program has a proven track record for reaching middle and high school students in the Los Angeles Unified School District, most of whom are economically disadvantaged Hispanic, African-American or other ethnic minorities. In cooperation with COSEE-West, the research and education teams will develop modules to add to existing teacher enhancement and student enrichment projects. Finally, all data will be summarized on a dedicated San Pedro Ocean Time Series website with links from all team member websites.

基因组序列的可用性为理解微生物的作用和生活如何以及它们如何与环境相互作用为基础。海洋微生物被认为代表了最伟大的生物多样性来源之一。 然而，研究海洋原核生物微生物（例如细菌）的子学科比最近研究的单细胞真核生命形式更为进步。由于最近的许多协同研究工作，可获得大型且成熟的原核生物数据库，而海洋微观真核生物的基线基因组信息远不如先进。 经常使用的分子方法来评估分类学多样性，提供了有限的有关已确定生物的生态作用或功能的信息。此处描述的程序旨在获得大量与已知生物相关的详细DNA序列数据。 将从王国Euglenozoa，Alveolata，Stramenopila和Cercozoa获得四个先前研究的海洋分类单元的数据。这些更详细的数据将显着增加有关公共领域环境微生物真核生物的基因组信息，并开始详细研究原核生物和真核生物之间的关系。该采样将在圣佩德罗频道的南加州大学（USC）良好的海洋学时间系列站进行，研究团队将可以访问同一站点工作的其他科学家收集的丰富生物学和化学数据。 这项研究具有巨大的希望，可以释放海洋微生物研究领域的研究，并将有助于促进海洋微生物学领域和海洋元基因组学领域的知识状态。在基础研究中，该项目还将产生一些更广泛的教育影响。基因组学是一个迅速发展的领域，通常被一般人群误解。研究产生的信息将增强教学课程，并为本科和研究生级研究提供机会。研究小组将与USC箭牌环境研究研究所（WIES）教育者合作，致力于通过灰色受众为K开发基于广泛的教育外展计划。 WEIS计划是一个非凡的教育平台，理想情况下适合增强其现有计划。研究团队还将直接参加由USC Wrigley Institute，UCLA和几个非正式科学合作伙伴开设的正在进行的教师教育增强计划（NSF资助的海洋科学教育卓越中心； Cosee-West）。 Cosee-West计划在洛杉矶统一学区的中学和高中生方面拥有良好的记录，其中大多数在经济上处于不利地位的西班牙裔，非裔美国人或其他少数民族。 与Cosee-West合作，研究和教育团队将开发模块，以增加现有的教师增强和学生丰富项目。最后，所有数据将在专门的San Pedro海洋时间序列网站上汇总，并附上所有团队成员网站的链接。