Lipid Dip-Pen Nanolithography for Model Bio-Membrane Systems

用于模型生物膜系统的脂质浸笔纳米光刻

• 批准号: 0723542
• 负责人: Chad Mirkin
• 金额: $ 42万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0723542&HistoricalAwards=false
• 关键词: Lipid; Dip; Pen; Nanolithography; Model

The Analytical and Surface Chemistry Program in the Division of Chemistry will support the collaborative research program of Prof. Mirkin of Northwestern University and Prof. Harald Fuchs of the University of Munster, Germany. This award coordinates with a collaborative award funded by the Deutsche Forschungsgemeinschaft (DFG) through a joint program between the NSF and DFG that jointly funds collaborative projects between US and German investigators.The purpose of this collaborative project is to develop the method of Lipid Dip-Pen Nanolithography (L-DPN) for the fabrication of phospholipid membranes capable of mimicking cellular functions in vitro. The expertise of the Mirkin group in surface biochemistry, DPN and microfabrication will be combined with the Fuchs group expertise in scanning probe microscopy, self organized systems and nano-scale analytics. There is growing evidence that the various properties of biological membranes strongly depend on both two- and three-dimensional heterogeneities. Therefore, it would be useful for modeling cellular functions to have multi-component membrane arrays with ultra-high lateral resolution in which 2 and 3 dimensional interaction can be controlled. Current methods for generating fluid and mechanically stable membrane arrays on surfaces are not optimum for patterning lipids on substrates for reconstitution of proteins. Additionally, encapsulation of materials into supported lipid multilayers at an appropriate scale or with several chemical components remains a significant challenge. DPN is the only currently available technology that may be capable of overcoming these obstacles. L-DPN fabricated patterns will be characterized as a function of the lipid ink composition, substrate chemistry, and environmental conditions in order to determine suitable feature sizes for studying interactions of biological molecules, the supramolecular structures of lipid patterns, and materials which are amenable to integration with phospholipid inks. Once fully developed, these tools will allow biologists, chemists, and others to study biological and chemical interactions at a fundamental level. The project will provide excellent training opportunities to students in a highly multi-disciplinary area, which will include international research experience in Germany and for German students in the US.The award is co-funded by the Office of International Science and Engineering at NSF.

化学系的分析与表面化学项目将支持西北大学Mirkin教授和德国明斯特大学Harald Fuchs教授的合作研究项目。 该奖项与德国研究协会 (DFG) 通过 NSF 和 DFG 之间的联合项目资助的合作奖项相协调，该项目共同资助美国和德国研究人员之间的合作项目。该合作项目的目的是开发脂质浸入方法笔式纳米光刻 (L-DPN) 用于制造能够模拟体外细胞功能的磷脂膜。 Mirkin 团队在表面生物化学、DPN 和微加工方面的专业知识将与 Fuchs 团队在扫描探针显微镜、自组织系统和纳米级分析方面的专业知识相结合。越来越多的证据表明，生物膜的各种特性强烈依赖于二维和三维异质性。 因此，拥有超高横向分辨率的多组件膜阵列（其中可以控制 2 维和 3 维相互作用）对于细胞功能建模将非常有用。目前在表面上生成流体和机械稳定膜阵列的方法对于在基质上形成脂质图案以重构蛋白质来说并不是最佳的。此外，以适当的规模或用多种化学成分将材料封装到支撑的脂质多层中仍然是一个重大挑战。 DPN 是目前唯一能够克服这些障碍的技术。 L-DPN 制造的图案将根据脂质墨水成分、基材化学和环境条件进行表征，以确定合适的特征尺寸，用于研究生物分子的相互作用、脂质图案的超分子结构以及适合的材料与磷脂墨水整合。一旦完全开发出来，这些工具将使生物学家、化学家和其他人能够在基础水平上研究生物和化学相互作用。该项目将为高度多学科领域的学生提供极好的培训机会，其中包括在德国和在美国的德国学生的国际研究经验。该奖项由美国国家科学基金会国际科学与工程办公室共同资助。