MRI: Acquisition of Instuments to Facilitate Molecular-Level Studies in Earth and Ocean Sciences

MRI：购置仪器以促进地球和海洋科学的分子水平研究

• 批准号: 0723222
• 负责人: Lihini Aluwihare
• 金额: --
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0723222&HistoricalAwards=false
• 关键词: MRI; Acquisition; Instuments; Facilitate; Molecular

The proposal requests funds for a gas-chromatograph time-of-flight mass spectrometer with a preparatory fraction collector and a liquid chromatograph-linear ion trap mass spectrometer with multiple sample ionization modes and an analytical fraction collector. The instrumentation will be used by the PI and her students to characterize and purify biomolecules from complex environmental samples for chemical, biological and geological research efforts. The facility will be used to support existing and proposed research efforts at SIO, including identification of membrane lipid and protein modifications associated with temperature and pressure tolerance of deep-ocean bacteria, and genomics enabled studies of membrane lipid biosynthesis. The facility will also be used to develop new analytical methods, and train graduate students in instrumentation operation and use. Use of the facility by outside users is by invitation.Broader impactsThe PI has a strong record of enhancing the participation of women in science by hosting female high school students. The proposed instrumentation will complement and expand the capabilities of SIO researchers, as well as invited outside researchers. Seminars will be held for either the Marine Chemistry and Geochemistry or Marine Biology seminar series demonstrating the facility capabilities and results to the larger SIO populace

该提案要求为配备预备级分收集器的气相色谱飞行时间质谱仪和配备多种样品电离模式和分析级分收集器的液相色谱-线性离子阱质谱仪提供资金。 首席研究员和她的学生将使用该仪器来表征和纯化复杂环境样本中的生物分子，以进行化学、生物和地质研究工作。 该设施将用于支持 SIO 现有和拟议的研究工作，包括鉴定与深海细菌的温度和压力耐受性相关的膜脂和蛋白质修饰，以及基因组学支持膜脂生物合成的研究。 该设施还将用于开发新的分析方法，并培训研究生仪器操作和使用。 外部用户通过邀请使用该设施。更广泛的影响 PI 在通过接待女高中生来提高女性对科学的参与方面有着良好的记录。 拟议的仪器将补充和扩展 SIO 研究人员以及受邀外部研究人员的能力。 将举办海洋化学和地球化学或海洋生物学系列研讨会，向更多 SIO 民众展示设施的能力和成果