Collaborative Research: Characterizing microbial transformation of marine DOM at the molecular level using untargeted metabolomic

合作研究：利用非靶向代谢组学在分子水平上表征海洋 DOM 的微生物转化

• 批准号: 2023509
• 负责人: Lihini Aluwihare
• 金额: $ 48.51万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2023509&HistoricalAwards=false
• 关键词: Collaborative; Research; Characterizing; microbial; transformation

Collaborative Research: Characterizing microbial transformation of marine dissolved organic matter at the molecular level using untargeted metabolomicsDissolved organic matter is an important component of the global carbon cycle. Dissolved organic matter provides food and energy for microbes living in the ocean and influences microbial diversity. Microbes convert some dissolved organic matter to CO2 (respiration) whereas other forms of dissolved organic matter are altered by microbial processes and persist in the ocean. Thus, it is important to understand how microbes change dissolved organic matter composition and reactivity. This project will examine the chemical structure of dissolved organic matter to identify: 1) molecules that fulfill carbon demand (biomass produced minus losses from respiration) and 2) transformation processes that result from microbial activity. The project will combine lab experiments and field studies at the Moorea Coral Reef Long Term Ecological Research site. The project will support training for three graduate students in marine biogeochemistry. Undergraduate training is aimed at sustained mentoring of underrepresented minority (URM) students. Undergraduates will be recruited from existing programs at Minority Serving Institutions at San Diego State University and the University of Hawaiʻi at Mānoa. Undergraduates will participate in the Scripps Institution of Oceanography SURF Research Experiences for Undergraduates program, where they will conduct research in marine chemistry. The goal is to provide a mentoring approach that can successfully overcome roadblocks to URM engagement in STEM and increase retention of these students in marine science.This work will combine field and lab studies using advanced molecular-level chemical characterization tools to explore how bacteria alter the composition and bioreactivity of organic compounds dissolved in seawater. Additionally, this project will develop informatics-based tools to identify a larger proportion of chemical structures in marine dissolved organic matter (DOM) than is currently possible using traditional approaches. The project will use tandem mass spectrometry and networking techniques to comprehensively classify organic compounds into molecular families and determine common chemical transformations. Then, using a well-developed field-based experimental ecosystem to produce diverse labile DOM pools the research team will track microbial transformation using expression of hydrolytic enzymes and measure selection for particular microbial taxa and metabolisms. This approach defines the reactivity of individual molecules and broader compound classes participating in carbon fluxes that underpin DOM-microbe interactions. Field surveys conducted within the Moorea Coral Reef Long Term Ecological Research program will explore methods to track transformation of specific molecules in the environment and validate experimental observations of compound classes that appear to accumulate as semi-labile DOM. By integrating laboratory and field experiments and oceanographic surveys with the refinement of analytical tools for untargeted metabolomics, this project will characterize the fate of reactive DOM in the ocean.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

合作研究：利用非靶向代谢组学在分子水平上表征海洋溶解有机物的微生物转化溶解有机物是全球碳循环的重要组成部分溶解有机物为海洋中的微生物提供食物和能量并影响微生物的多样性。一些溶解的有机物转化为二氧化碳（呼吸），而其他形式的溶解的有机物则被微生物过程改变并持续存在于海洋中。因此，了解微生物如何改变溶解的有机物非常重要。该项目将检查损失的溶解有机物的化学结构，以确定：1）满足碳需求的分子（呼吸作用产生的生物量减去）和2）微生物活动产生的转化过程。该项目将支持对三名海洋生物地球化学研究生的培训，旨在持续指导少数族裔 (URM) 学生。本科生将从圣地亚哥州立大学和夏威夷大学马诺阿分校的现有项目中招募，本科生将参加斯克里普斯海洋学研究所 SURF 本科生研究经验项目，在那里他们将进行海洋化学研究。旨在提供一种指导方法，可以成功克服 URM 参与 STEM 的障碍，并提高这些学生对海洋科学的保留率。这项工作将使用先进的分子级化学将现场研究和实验室研究结合起来此外，该项目将开发基于信息学的工具来识别海洋溶解有机物（DOM）中比目前使用传统方法更大比例的化学结构。该项目将使用串联质谱和网络技术将有机化合物全面分类为分子家族，并确定常见的化学转化，然后，研究团队将使用成熟的现场实验生态系统来生产各种不稳定的 DOM 库。转型使用水解酶的表达并测量特定微生物类群和代谢的选择，该方法定义了参与碳通量的单个分子和更广泛的化合物类别，这些碳通量支持莫雷阿岛珊瑚礁长期生态研究中进行的 DOM-微生物相互作用。该计划将探索跟踪环境中特定分子转化的方法，并通过将实验室和现场实验以及海洋学调查与分析工具的改进相结合，验证以半不稳定 DOM 形式积累的化合物类别的实验观察结果。该项目将描述海洋中反应性 DOM 的命运。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Molecular Commerce on Coral Reefs: Using Metabolomics to Reveal Biochemical Exchanges Underlying Holobiont Biology and the Ecology of Coastal Ecosystems

珊瑚礁上的分子商务：利用代谢组学揭示全生物生物学和沿海生态系统生态学背后的生化交换

• DOI: 10.3389/fmars.2021.630799
• 发表时间: 2021-07
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: Wegley Kelly, Linda;Nelson, Craig E.;Aluwihare, Lihini I.;Arts, Milou G.;Dorrestein, Pieter C.;Koester, Irina;Matsuda, Shayle B.;Petras, Daniel;Quinlan, Zachary A.;Haas, Andreas F.
• 通讯作者: Haas, Andreas F.

ConCISE: Consensus Annotation Propagation of Ion Features in Untargeted Tandem Mass Spectrometry Combining Molecular Networking and In Silico Metabolite Structure Prediction

ConCISE：结合分子网络和计算机模拟代谢物结构预测的非靶向串联质谱中离子特征的一致性注释传播

• DOI: 10.3390/metabo12121275
• 发表时间: 2022-12
• 期刊: Metabolites
• 影响因子: 4.1
• 作者: Quinlan, Zachary A.;Koester, Irina;Aron, Allegra T.;Petras, Daniel;Aluwihare, Lihini I.;Dorrestein, Pieter C.;Nelson, Craig E.;Wegley Kelly, Linda
• 通讯作者: Wegley Kelly, Linda