NER: Actin Filament Crosslinking in vitro and in vivo using Purified and Isolated Carbon Nanotubes ? A Potential Cancer Therapy

NER：使用纯化和分离的碳纳米管进行体外和体内肌动蛋白丝交联？

• 批准号: 0708418
• 负责人: Kris Dahl
• 金额: --
• 依托单位: Carnegie-Mellon University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0708418&HistoricalAwards=false
• 关键词: NER; Actin; Filament; Crosslinking; vitro

0708418 Dahl This proposed research will establish a relationship between subcellular reorganization and toxicological effects to incorporation of anisotropic molecules into cells. in light of conflicting evidence of the CNT toxicity, the PIs will undertake careful study of the interaction of well-characterized and purified CNTs with live cells. The CNT-cell interactions will be closely monitored spectroscopically and microscopically from the point of entry into the cell. The results will launch the development of novel nanomaterials for cellular therapies as well as deepen the understanding of cytoskeletal reorganization cellular functions. After analyzing and understanding the effects of CNTs on cell behavior, the investigators will modify the large surface areas of CNTs to design and develop non-destructive targeted cancer therapies.

0708418 DAHL这项提出的研究将建立亚细胞重组与毒理学效应与将各向异性分子掺入细胞之间的关系。鉴于CNT毒性的证据相互矛盾，PI将仔细研究良好的和纯化的CNT与活细胞的相互作用。 CNT细胞相互作用将从进入细胞点从光谱和显微镜进行密切监测。结果将启动用于细胞疗法的新型纳米材料的开发，并加深对细胞骨架重组细胞功能的理解。在分析和理解CNT对细胞行为的影响之后，研究人员将修改CNT的较大表面积，以设计和开发非破坏性的靶向癌症疗法。