IDBR: Development of High-Speed Two-Photon Excitation Fluorescence Microscopy with Chromatically Extended Depth of Focus

IDBR：开发具有彩色扩展焦深的高速双光子激发荧光显微镜

• 批准号: 0649866
• 负责人: Zhiwen Liu
• 金额: $ 43.57万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649866&HistoricalAwards=false
• 关键词: IDBR; Development; Speed; Two; Photon

This award is for the development of a faster two-photon fluorescence microscope, with improvements especially in the axial scanning speed. Speed will be increased by wavelength division multiplexing (or chromatic two-photon imaging). Although much progress has been made in improving the lateral scanning speed, a high-speed axial scanning method is still missing. Slow mechanical translation of the objective lens or the specimen itself is still required in order to scan in the axial direction, which limits the 3D imaging speed. As a result, existing instruments have very limited capability to monitor fast biological processes (e.g., transient calcium signals) in 3D and in the x-z or y-z cross-section. The proposed instrument will have a significant impact on 3D microscopy, one of the most important and widely used tools in biology. It will allow researchers to study fast dynamics (e.g., membrane trafficking, chemical waves within or between cells) and provide fundamental understanding of various inter- and intra- cellular processes such as calcium imaging. In addition to its scientific benefits, the project will involve undergraduate researchers as well as graduate students. Woman and minority students will be particularly encouraged to be involved in the program. The investigators will work closely with Penn State organizations such as Women in Engineering to develop outreach education programs for under-represented groups as well as K-12 students.

该奖项旨在表彰开发速度更快的双光子荧光显微镜，特别是在轴向扫描速度方面的改进。 通过波分复用（或彩色双光子成像）可以提高速度。尽管在提高横向扫描速度方面已经取得了很大进展，但仍然缺乏高速轴向扫描方法。为了沿轴向扫描，仍然需要物镜或样本本身缓慢的机械平移，这限制了 3D 成像速度。因此，现有仪器在 3D 和 x-z 或 y-z 横截面中监测快速生物过程（例如瞬态钙信号）的能力非常有限。 拟议的仪器将对 3D 显微镜产生重大影响，3D 显微镜是生物学中最重要和最广泛使用的工具之一。 它将允许研究人员研究快速动力学（例如，膜运输、细胞内或细胞间的化学波），并提供对各种细胞间和细胞内过程（如钙成像）的基本了解。除了其科学效益外，该项目还将涉及本科生研究人员和研究生。我们将特别鼓励女性和少数民族学生参与该计划。调查人员将与宾夕法尼亚州立大学的组织（例如工程女性）密切合作，为代表性不足的群体以及 K-12 学生制定外展教育计划。