Acquisition of a 600 MHz Cryogenic Probe for Research and Education for the NMR Facility at the City College of New York

为纽约城市学院的 NMR 设施采购用于研究和教育的 600 MHz 低温探头

• 批准号: 0619224
• 负责人: Ranajeet Ghose
• 金额: $ 38.66万
• 依托单位: CUNY City College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0619224&HistoricalAwards=false
• 关键词: Acquisition; 600; MHz; Cryogenic; Probe

This award is for the acquisition of a cryogenic probe for a 600 MHz NMR spectrometer housed in the Magnetic Resonance Facility at the college. The cryogenic probe will afford a 3-4-fold enhancement in sensitivity compared with conventional triple-resonance probes. In addition, being "13C-enhanced", it will greatly improve capabilities for 13C direct detection with sensitivities comparable to dedicated 13C-detect probes, allowing additional experimental methodology for biomolecules to be developed and implemented. Further, the new salt-tolerant design will prevent a significant degradation of the sensitivity gain in high-salt conditions that are often necessary to stabilize biomolecules in solution. The availability of this probe will allow faster data acquisition and/or analysis of samples that cannot be produced at high concentrations. The probe will benefit a broad spectrum of research areas including (i) the development of novel NMR methodology to study biomolecular structure and dynamics, (ii) determination of structure-function relationships in flavoproteins, (iii) investigation of charge effects in ordered and disordered proteins and (iv) the elucidation of the molecular determinants of membrane protease function. The City College of New York is a minority-serving institution. The availability of the cryogenic probe at the NMR facility will allow the training of a larger number of students (graduate and undergraduate) and postdoctoral researchers, including those belonging to communities that are underrepresented in the sciences. There are plans to develop an NMR workshop that would bridge the gap between theory and "hands-on" experimentation. This would be beneficial both for the college students and for the state's structural biology community in general.

该奖项是因为收购了该学院磁共振设施中容纳600 MHz NMR光谱仪的低温探针。与传统的三个共和度探针相比，低温探针的灵敏度增强了3-4倍。此外，作为“ 13C增强”，它将大大提高13C直接检测的能力，其灵敏度可与专用的13C检测探针相当，从而可以开发和实施生物分子的其他实验方法。此外，新的耐盐设计将防止在溶液中稳定生物分子所必需的高盐条件下的敏感性增长的显着降解。该探针的可用性将允许更快地获取数据和/或分析无法在高浓度下产生的样品。该探测器将受益于广泛的研究领域，包括（i）研究新型NMR方法论，以研究生物分子结构和动力学，（ii）确定黄素蛋白中结构功能关系的确定，（iii）在有序和无序蛋白质和（iv）强调分子蛋白质蛋白质蛋白质蛋白质中的收费效应。纽约城市学院是少数派服务机构。 NMR设施的低温探测器的可用性将允许培训更多的学生（研究生和本科）和博士后研究人员，包括属于科学中代表性不足的社区的学生。有计划开发一个NMR研讨会，该研讨会将弥合理论和“动手”实验之间的差距。这对大学生和该州的结构生物学社区都将是有益的。