Thermodynamic Modeling of Fluorescent In Situ Hybridization (FISH) for Environmental Applications

环境应用荧光原位杂交 (FISH) 的热力学模型

• 批准号: 0606894
• 负责人: Daniel Noguera
• 金额: --
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0606894&HistoricalAwards=false
• 关键词: Thermodynamic; Modeling; Fluorescent; Situ; Hybridization

0606894NogueraThe proposed research continues the PI's previous research into a thermodynamics-based model to predict formamide dissociation curves, a key step in use of the FISH protocol, a molecular biology tool widely used in microbial identification studies. The concept was previously demonstrated for E. coli; the next step is to demonstrate the applicability of the same modeling approach to five other diverse bacteria/archea. If the work is successful, this project could significantly improve the efficiency of designing FISH probes and have widespread beneficial impacts. The proposal includes a plan to create an Internet tool to make results of the project available researchers in microbial ecology and environmental engineering.

0606894Noguerathe提出的研究继续PI先前对基于热力学的模型的研究来预测甲酰胺分离曲线，这是FISH方案的使用的关键步骤，这是一种分子生物学工具，一种广泛用于微生物识别研究。以前为大肠杆菌证明了这个概念。下一步是证明对其他五种其他不同细菌/古生的建模方法的适用性。如果工作成功，该项目可以显着提高设计鱼类探针的效率并产生广泛的有益影响。该提案包括一个计划，以创建一个互联网工具，以使项目研究人员在微生物生态学和环境工程领域获得可用的结果。