Microbiological and Biochemical Analysis of Phosphorus Accumulating Organisms in Full Scale Wastewater Treatment Plants

大型污水处理厂中磷积累生物的微生物和生化分析

• 批准号: 9909103
• 负责人: Daniel Noguera
• 金额: $ 35.37万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-15 至 2005-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9909103&HistoricalAwards=false
• 关键词: Microbiological; Biochemical; Analysis; Phosphorus; Accumulating

9909103 Noguera The objective of research being conducted with support by this award is to investigate the microbiology and biochemistry of phosphate removal in full-scale wastewater treatment plants as steps toward improvement in operation of biotreatment processes for enhanced removal of phosphates from wastewater. Laboratory work involves application of a technique based on differences of buoyant densities between cells of microorganisms that accumulate phosphates from those that do not accumulate them as a physical method for their separation and isolation. In this work, a procedure will be used for visualization of polyphosphate staining in combination with fluorescent hybridization to allow simultaneous identification of microorganisms and determination of their accumulations of polyphosphates. Results of laboratory-scale research will be correlated with knowledge obtained from studies of the microbial phosphorus accumulating communities of wastewater treatment plants located in Madison, Stoughton and Racine, Wisconsin. the wastewater treatment plant in Madison uses a modified UCT (University of Cape Town) biotreatment process, the Stoughton plant operates an advanced oxidation process and the Racine wastewater treatment plant uses a conventional suspended microbial growth, non-enhanced biological phosphate removal process. Results of this research are expect to relate knowledge obtained to improvement in the engineering design and operation of treatment plants and systems that utilize metabolic activities of microorganisms to remove plant nutrients from wastewater. This work is complementary to research being conducted with support from NSF Grant No. 9875642, the objective of which is to investigate the microbial ecology of nitrogen removal from wastewater by aerated-anoxic processes. ***

9909103 Noguera在该奖项的支持下进行研究的目的是研究全面废水处理厂中磷酸盐去除的微生物学和生物化学，以改善生物治疗过程的步骤，以增强从废水中磷酸盐去除的磷酸盐。 实验室工作涉及基于微生物细胞之间浮力密度差异的技术应用，这些微生物细胞从不积聚它们作为分离和分离的物理方法的磷酸盐中积累了磷酸盐。 在这项工作中，将使用一项程序可视化多磷酸染色与荧光杂交结合使用，以同时鉴定微生物并确定它们的多磷酸盐的积累。 实验室规模研究的结果将与来自位于麦迪逊，斯托顿和威斯康星州拉辛的废水处理厂的微生物磷的研究获得的知识相关。 麦迪逊的废水处理厂使用改良的UCT（开普敦大学）生物处理过程，Stoughton植物经营先进的氧化过程，Racine废水处理厂使用常规的悬浮微生物生长，非增强生物学磷酸盐去除过程。 这项研究的结果有望将获得的知识与改善处理厂的工程设计和运营相关联，这些处理厂和系统利用微生物的代谢活动来从废水中清除植物营养素。 这项工作与NSF No. 9875642的支持进行了研究，其目的是通过充气 - 氧化过程研究从废水中去除氮的微生物生态学。 ***