Enhancing Productivity and Protein Quality in Perfusion Cultures

提高灌注培养的生产力和蛋白质质量

• 批准号: 0541119
• 负责人: Dhinakar Kompala
• 金额: --
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0541119&HistoricalAwards=false
• 关键词: Enhancing; Productivity; Protein; Quality; Perfusion

0541119KompalaThe therapeutic efficacy of a biopharmaceutical protein is extremely sensitive to the production conditions because they influence post-translation modifications. Therefore, any new concept for production of such proteins used in humans requires thorough characterization of its impact on the integrity of the product. The proposed study will investigate the author's hypothesis that slow growing perfusion cultures produce more therapeutically relevant glycoproteins compared to those produced in faster growing batch cultures. This proposal addresses an issue that has profound consequences from the practical perspective of advancing the development of an important new class of therapeutic agents (i.e., recombinant glycoproteins). Success in this area will have a significant financial payoff. In addition, from the perspective of "advancing knowledge" this proposal also has as one of its aims the goal of better understanding the underlying biological processes that determine glycosylation patterns.

0541119 kompalathe生物药物蛋白的治疗功效对生产条件非常敏感，因为它们会影响翻译后修饰。因此，任何用于人类中使用的蛋白质生产的新概念都需要彻底表征其对产品完整性的影响。拟议的研究将调查作者的假设，即与在更快的生长批量培养物中相比，与生长较快的糖蛋白相比，缓慢生长的灌注培养物会产生更具治疗性相关的糖蛋白。 该提议从实际的角度来促进了重要的新型治疗剂（即重组糖蛋白）的开发，从而解决了一个问题。这一领域的成功将带来巨大的财务回报。此外，从“促进知识”的角度来看，该提案的目标之一是更好地理解决定糖基化模式的基本生物学过程的目标之一。