Engineering CHO Cells to Achieve a High-Level Inverse-Growth-Associated Production of Secreted Glycoproteins in High Cell Density Cultures

改造 CHO 细胞以在高细胞密度培养物中实现分泌糖蛋白的高水平逆生长相关生产

• 批准号: 9817249
• 负责人: Dhinakar Kompala
• 金额: $ 38.79万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9817249&HistoricalAwards=false
• 关键词: Engineering; CHO; Cells; Achieve; Level

The objective of this project is to increase the efficiency of cell culture systems by; (a) genetic manipulation of the cells; and (b) utilization of a cell retention system. Genetic manipulation utilizing an AML promoter specific to the G1-phase adds to the benefits of increasing the specific production rate at lower growth rates. Under these conditions, cells can be cultured at low media exchange rates and a higher titer can be achieved. Utilization of a cell retention system increases the cell density and allows a higher volumetric productivity. The CHO expression system considered in this proposal is extensively used in industry and the results from this research will be very applicable to industrial manufacturing.

该项目的目的是提高细胞培养系统的效率； （a）细胞的遗传操纵； （b）使用细胞保留系统。 利用AML启动子特异性G1相的遗传操作增加了以较低生长速率提高特定生产率的好处。 在这些条件下，可以以低介质汇率培养细胞，并可以实现更高的滴度。 细胞保留系统的利用会增加细胞密度并允许更高的容量生产率。 该提案中考虑的CHO表达系统在行业中广泛使用，这项研究的结果将非常适用于工业制造业。