Presidential Young Investigator Award: Design and Strategies for Optimizing Bioprocesses

总统青年研究员奖：优化生物过程的设计和策略

• 批准号: 8857719
• 负责人: Dhinakar Kompala
• 金额: $ 34.2万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8857719&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Design

This research covers a variety of important problems in the field of biotechnology; however, the major emphasis is focussed on studies of recombinant bacterial cultures, the elimination of plasmid instability, and the overproduction of recombinant products. Both batch-fed and continuous cultures are being examined with the ultimate goal of achieving optimal process control of the systems. Affinity cell separation techniques will be used to increase the longevity of recombinant bacterial cultures. It is hoped that this can be accomplished by the selective removal of plasmid-free segregants. Also, technolgy for maximizing hybridoma cell production of monoclonal antibodies and the use of high-voltage electric pulses for the electrofusion of animal cells are being investigated. The primary thrust of this work is to improve bioreactor performance for the manufacture of economic bioproducts. The result that is desired is that many biologicals that can only be produced in micro quantities today will be manufactured in kilogram or greater quantities in the near future. This achievement would make bioproducts available at affordable cost and, therefore, contribute to an improved standard of living for the general populous.

这项研究涵盖了生物技术领域的各种重要问题。但是，主要重点集中在重组细菌培养物，消除质粒不稳定性以及重组产物过多的研究上。 批处理和连续培养物都以实现系统最佳过程控制的最终目标进行了检查。 亲和细胞分离技术将用于增加重组细菌培养物的寿命。 希望这可以通过选择性去除无质粒的隔离剂来实现。 同样，正在研究为最大化单克隆抗体杂交瘤细胞产生的技术，并正在研究使用高压电脉冲进行动物细胞的电催化。 这项工作的主要目的是改善生物反应器的性能，用于生产经济生物产品。 所需的结果是，今天只能以微量量生产的许多生物学物质将在不久的将来以千克或更大的数量制造。 这项成就将使生物产品以负担得起的成本提供，因此为普通人口增长了改善的生活水平。