Dissertation Research: Comparative Genomics: A Novel Approach to Understanding Mammalian Reproductive Proteins

论文研究：比较基因组学：理解哺乳动物生殖蛋白的新方法

• 批准号: 0508908
• 负责人: Mark Springer
• 金额: --
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0508908&HistoricalAwards=false
• 关键词: Dissertation; Research; Comparative; Genomics; Novel

Portions of three male and two female candidate reproductive proteins believed to play a role in sperm and egg extracellular matrix interactions will be sequenced from a diverse range of placental mammals. This extensive database will be used in conjunction with molecular analyses to identify amino acid sites that are evolving rapidly and hence may be important in protein interactions. This rapid evolution will also be used to detect proteins or sites that may be coevolving. In addition, the database will be used to search for regions of conservation that may be important in protein structure and function.Characterizing these proteins and identifying their interactions will have a broader impact on diagnosing causes of infertility, understanding protein interactions conferring species-specificity and expanding the knowledge of protein co-evolution, which can be utilized in many other fields such as cancer research. This proposal presents a novel approach that takes advantage of the power of comparative genomics to characterize the proteins involved in mammalian fertilization. This project will afford opportunities for undergraduate volunteer participation in this exciting new bioinformatics field.

三种雄性和两种雌性候选生殖蛋白的部分被认为在精子和卵细胞外基质相互作用中发挥作用，将对多种胎盘哺乳动物进行测序。 这个广泛的数据库将与分子分析结合使用，以识别快速进化的氨基酸位点，因此可能对蛋白质相互作用很重要。这种快速进化也将用于检测可能共同进化的蛋白质或位点。此外，该数据库将用于搜索对蛋白质结构和功能可能重要的保守区域。表征这些蛋白质并识别它们的相互作用将对诊断不孕原因、了解赋予物种特异性的蛋白质相互作用和扩展蛋白质共同进化的知识，可用于癌症研究等许多其他领域。 该提案提出了一种新方法，利用比较基因组学的力量来表征哺乳动物受精中涉及的蛋白质。 该项目将为本科生志愿者提供参与这个令人兴奋的新生物信息学领域的机会。