PostDoctoral Research Fellowship in Polar Regions

极地博士后研究奖学金

• 批准号: 0443754
• 负责人: Bradley Buckley
• 金额: $ 12.92万
• 依托单位: Buckley Bradley A
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-11-01 至 2006-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0443754&HistoricalAwards=false
• 关键词: PostDoctoral; Research; Fellowship; Polar; Regions

This award is for a postdoctoral fellowship. The investigator will use molecular tools study the reasons why antarctic fish are unable to tolerate water warmer than about 6oC. These fish are extreme stenotherms, having evolved in sub-zero degree waters for somewhere between 5-15 million years. It is not known at this time whether the inability of these fish to tolerate increases in temperature is reflected in an inability to shift gene expression in response to heat stress. The proposed experiments will create the first cDNA (complementary DNA) microarray from Trematomus bernacchii, a common species in McMurdo Sound. The tissues of fish acclimatized over varying time periods to 4oC will be used to construct a complex, multi-tissue cDNA library of genes expressed during heat exposure. This library will be used to fabricate a cDNA microarray that will allow us to monitor the simultaneous expression of thousands of genes. RNA from control and heat shocked individuals will be used to create fluorescently tagged cDNA probes to be hybridized to the microarray, thereby generating gene expression profiles for thermally responsive genes. The primary hypothesis is that the ability to shift gene expression in response to elevated temperature will be absent in T. bernacchii and two related Antarctic species. The gene expression patterns from the Antarctic species will be compared to their temperate relatives from New Zealand. This project will increase our understanding of the genomic mechanisms related to cold-adaptation, stenothermality, and the response of polar fauna to environmental change.

该奖项是因为博士后奖学金。 研究人员将使用分子工具研究南极鱼无法耐受水温暖的原因，而不是6oC。 这些鱼是极端的stehotherms，在零度的水域中进化了5 - 1500万年。目前尚不清楚这些鱼是否无法忍受温度升高，反映出无法响应热应激而转移基因表达。 提出的实验将创建来自McMurdo Sound中常见物种Trematomus bernacchii的第一个cDNA（互补DNA）微阵列。 在变化的时间段内适应4oC的鱼类的组织将用于构建热暴露期间表达的复杂的，多组织的基因库。该库将用于制造cDNA微阵列，该微阵列将使我们能够同时监测数千个基因的表达。来自对照和热震动个体的RNA将用于创建荧光标记的cDNA探针，以杂交与微阵列，从而为热响应基因生成基因表达谱。主要假设是，在伯纳奇奇（T. bernacchii）和两个相关的南极物种中，将不存在响应升高温度的基因表达的能力。来自南极物种的基因表达模式将与来自新西兰的温带亲戚进行比较。该项目将提高我们对与冷适应，steenothermality和Polar Fauna对环境变化的反应有关的基因组机制的理解。