The Cellular Stress Response in Cold-adapted Organisms: Building Novel Mechanistic Links between Heat Stress, Cell Cycle Arrest and Apoptosis in Antarctic Fishes.

适应寒冷的生物体的细胞应激反应：在南极鱼类的热应激、细胞周期停滞和细胞凋亡之间建立新的机制联系。

基本信息

批准号：
0944743
负责人：
Bradley Buckley
金额：
$ 46.45万
依托单位：
Portland State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2010
资助国家：
美国
起止时间：
2010-08-15 至 2013-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0944743&HistoricalAwards=false
关键词：
Cellular Stress Response Cold adapted

项目摘要

The research will investigate a novel mechanism by which cold-adapted fishes of the Southern Ocean sense and respond to elevated temperatures. It is hypothesized that sub-lethal heat stress may induce cell cycle arrest and/or programmed cell death through apoptosis. The study will use genome-enabled technologies to examine the environmental control over gene expression in Antarctic species and will build direct mechanistic links between the expression of a specific signaling pathway gene and heat-induced changes in cells. Prior results support the hypothesis that heat stress results in cell cycle arrest and, in some cases, programmed cell death in Antarctic fishes. If so, this represents a novel, modified version of the well-conserved cellular stress response found in essentially all other species and suggests that warming ocean temperatures may have profound cellular and physiological impacts on these extremely stenothermal species. The P.I. conducts outreach activities with the Oregon Museum of Science and Industry, will be involved in developing a science curriculum for the Native American Youth and Family Center (NAYA) Early College Academy in Portland, and supports the educational and professional development of both undergraduate and graduate students at Portland State University.

该研究将调查一种新型机制，通过这种机制，南大洋的冷适应鱼类感并响应升高的温度。假设亚致死性热应激可能通过细胞凋亡诱导细胞周期停滞和/或程序性细胞死亡。该研究将使用支持基因组的技术来检查南极物种中基因表达的环境控制，并将在特定信号通路基因的表达与热诱导的细胞变化之间建立直接的机械联系。先前的结果支持这样的假设，即热应激会导致细胞周期停滞，在某些情况下会导致南极鱼类中的细胞死亡。如果是这样，这代表了基本所有其他物种中保存良好的细胞应激反应的新颖，修改的版本，并表明温暖的海洋温度可能对这些极其稳固的物种产生深远的细胞和生理影响。 P.I.与俄勒冈州科学和工业博物馆进行外展活动，将参与波特兰的美国原住民青年和家庭中心（NAYA）早期学院的科学课程，并支持波特兰州立大学的本科生和研究生的教育和专业发展。