Integration von Expressions- und genomweiten Assoziations-Analysen in Kreuzungspopulationen kommerzieller Rassen zur Kartierung von QTL Regionen mit verbesserter Auflösung und funktionelle Analyse von Kandidatengenen für Wasserbindungsvermögen

在商业品种的杂交群体中整合表达和全基因组关联分析，以提高分辨率和水结合能力候选基因的功能分析来绘制 QTL 区域

基本信息

批准号：
168145480
负责人：
Professor Dr. Karl Schellander
金额：
--
依托单位：
Abteilung Tierzucht und Tierhaltung
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/168145480?language=en
关键词：
Integration von Expressions und genomweiten

项目摘要

Variability in meat quality traits like water holding capacity (WHC) is a major concern for producers and consumers. In the first phase of `DRIP´ we have already shown that many biological pathways including extracellular matrix receptor interaction, calcium signalling, oxidative phosphorylation, mitochondrial pathways, actin cytoskeleton signalling, PDGF signalling, EGF signalling, ERK/MAPK signalling, as well as TR/RXR activation are correlated with WHC. The combination of expression data with linkage studies offers new options to understand the biology at a global level and the genetic factors affecting the trait of interest. Expression-quantitative trait loci (eQTL) studies of genes with trait-correlated expression may provide the necessary information required for identifying genes that control quantitative phenotypes. We have identified a number of cis and trans eQTL that coincide with those of the QTL for drip loss (DRIP) identified in a F2 pig population. In order to refine these results additional selected F2 animals with maximized genetic and phenotypic dissimilarity will be used for expression profiling and genotyping. To overcome the weakness of a still limited mapping resolution within the F2-Families and to validate and confirm the biological pathways commercial populations will be used to validate and refine the results. Here, a whole genome association study for the muscle gene expression profiles of the commercial herd Pietrain x (German Landrace x German Large White) (Pix(DLxDE)) will be studied and the results obtained in this population will be compared with previously linkage studies in the F2 resource population. The list of candidate genes and linkage regions confirmed in both phase I and II will be used for further functional analysis.

保水能力 (WHC) 等肉类品质特性的变化是生产者和消费者关注的一个主要问题。在“DRIP”的第一阶段，我们已经证明了许多生物途径，包括细胞外基质受体相互作用、钙信号传导、氧化磷酸化、线粒体。表达数据与连锁研究的结合提供了与 WHC 相关的信号通路、肌动蛋白细胞骨架信号、PDGF 信号、EGF 信号、ERK/MAPK 信号以及 TR/RXR 激活。在全球水平上了解生物学和影响性状的遗传因素的新选择对具有性状相关表达的基因进行表达数量性状位点（eQTL）研究可能会提供识别控制数量表型的基因所需的必要信息。我们已经鉴定出许多顺式和反式 eQTL，它们与 F2 猪群体中鉴定的滴水损失 (DRIP) QTL 一致。为了完善这些结果，将额外选择具有最大遗传和表型差异的 F2 动物。为了克服 F2 家族内作图分辨率仍然有限的弱点，并验证和确认商业群体，将用于验证和完善结果。将研究商业牛群 Pietrain x（德国长白 x 德国大白）（Pix(DLxDE)）的肌肉基因表达谱，并将在该群体中获得的结果与之前在 F2 资源群体列表中的连锁研究进行比较。在第一阶段和第二阶段中确认的候选基因和连锁区域将用于进一步的功能分析。