SST: Ricin Quantification in Aqueous Media

SST：水介质中的蓖麻毒素定量

• 批准号: 0426170
• 负责人: Craig Grimes
• 金额: $ 30万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0426170&HistoricalAwards=false
• 关键词: SST; Ricin; Quantification; Aqueous; Media

0426170GrimesThis research is specifically aimed at developing a highly sensitive, specific ricin sensor platform which, with the intellectual foundation in place, can be readily extended to enable simultaneous identification of multiple biotoxins or pathogens in aqueous media (e.g. water, blood, serum). Also, the sensor platform is designed to be of relatively low cost enabling practical implementation of the resulting sensor technology. The fundamental and critical questions to be answered by this research are, can mass sensitive magnetoelastic sensors be modified to permit the quantification of protein biotoxins, and can these devices be packaged into a small hand-held sensor to aid in the rapid quantification of ricin concentrations? To answer these questions the following specific aims are proposed: (1) investigate, on a fundamental level, the binding of the ricin biotoxin to polymer thin films modified with biorecognition molecules (antibodies or GSLs). In preliminary work the investigators have accomplished this for both ricin and E. coli O157:H7. Here they will identify antibodies or GSLs which the model biotoxin (ricin) will bind, then immobilize these recognition molecules on the surface of poly(ethylene glycol) hydrogels or self-assembled monolayers, and study the binding of the model biotoxins to these modified surfaces, (2) develop techniques for patterning these biomolecule-modified polymer gels on magnetoelastic substrates and study the changes in resonance frequency induced in the magnetoelastic material by binding of the model toxins onto the sensor surface, and (3) integrate the recognition molecule-modified magnetoelastic sensors and appropriate electronics into a hand-held device that can be readily used to monitor biotoxin content in collected fluid samples within a disposable cartridge. While efforts in this proposal are strictly focused on ricin, the resulting sensor technology could be adaptable to other toxins.

0426170Grimes这项研究专门旨在开发一种高度灵敏、特异性的蓖麻毒素传感器平台，在具备适当的知识基础的情况下，该平台可以很容易地进行扩展，以能够同时识别水介质（例如水、血液、血清）中的多种生物毒素或病原体。此外，传感器平台的设计成本相对较低，能够实际实施最终的传感器技术。这项研究要回答的基本和关键问题是，是否可以修改质量敏感磁弹性传感器以定量蛋白质生物毒素，以及是否可以将这些设备封装到小型手持式传感器中以帮助快速定量蓖麻毒素浓度？为了回答这些问题，提出了以下具体目标：（1）从根本上研究蓖麻毒素生物毒素与生物识别分子（抗体或GSL）修饰的聚合物薄膜的结合。在初步工作中，研究人员已经针对蓖麻毒素和大肠杆菌 O157:H7 实现了这一目标。在这里，他们将识别模型生物毒素（蓖麻毒素）将结合的抗体或 GSL，然后将这些识别分子固定在聚（乙二醇）水凝胶或自组装单层的表面，并研究模型生物毒素与这些修饰表面的结合，（2）开发在磁弹性基底上对这些生物分子改性聚合物凝胶进行图案化的技术，并研究通过将模型毒素结合到传感器上而在磁弹性材料中引起的共振频率的变化(3) 将识别分子修饰的磁弹性传感器和适当的电子器件集成到手持设备中，该手持设备可轻松用于监测一次性盒内收集的流体样品中的生物毒素含量。虽然该提案的工作重点是蓖麻毒素，但由此产生的传感器技术可以适用于其他毒素。