SynDiv - Synthetic Biology of the Bacterial Cell Division

SynDiv - 细菌细胞分裂的合成生物学

• 批准号: 165233936
• 负责人: Professorin Dr. Petra Schwille
• 金额: --
• 依托单位: Forschungsgruppe Zelluläre und molekulare Biophysik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/165233936?language=en
• 关键词: SynDiv; Synthetic; Biology; Bacterial; Cell

This collaborative European project is aimed at the design of new biological, biophysical and nanotechnological strategies how to manipulate and thereby investigate key steps in bacterial cell division in living bacteria, and to reconstitute these mechanisms in a minimal system based on liposomes. The final goals are twofold: first, better understanding of the relevance of topological constraints on cell division and proliferation, and second, design of an evolvable minimal cell system comprising the essential components for controlled encapsulation and replication of genetic material, using the key players of the bacterial cell division machinery (divisome). Our team is highly complementary, consisting of: 1) an expert in E.coli cell biology, particularly the chromosome organization and segregation in the living cell (Sherratt, United Kingdom), 2) a biophysicist with a strong background of cell and membrane biophysics, employing giant vesicles as toolboxes for bottom-up biology (Schwille, Germany), and 3) a nanotechnologist who developed new concepts for bacterial shape and growth control on micro- and nanofluidic structures (Dekker, The Netherlands). By bringing together these expertises, we are convinced that we can build a powerful incubator for novel technology-driven synthetic biology on the basis of bacterial systems in Europe.

这个欧洲合作项目旨在设计新的生物、生物物理和纳米技术策略，如何操纵并研究活细菌中细菌细胞分裂的关键步骤，并在基于脂质体的最小系统中重建这些机制。最终目标有两个：首先，更好地理解拓扑约束对细胞分裂和增殖的相关性，其次，设计一个可进化的最小细胞系统，其中包括用于受控封装和复制遗传物质的基本组件，利用关键参与者细菌细胞分裂机制（分裂体）。我们的团队具有高度互补性，包括：1) 大肠杆菌细胞生物学专家，特别是活细胞中的染色体组织和分离专家（Sherratt，英国），2) 具有深厚细胞和膜生物物理学背景的生物物理学家，采用巨型囊泡作为自下而上生物学的工具箱（Schwille，德国），以及 3）一位纳米技术专家，开发了微纳米流体结构上细菌形状和生长控制的新概念（德克尔，荷兰）。通过汇集这些专业知识，我们相信我们可以在欧洲细菌系统的基础上建立一个强大的新技术驱动合成生物学孵化器。