CAREER: Development Plan for Optical / Nuclear Double Resonance Studies of Proteins and Inclusion Complexes Oriented in Liquid Crystalline Matrices

职业：液晶基质中蛋白质和包合物的光学/核双共振研究发展计划

基本信息

批准号：
0349255
负责人：
Boyd Goodson
金额：
$ 55.2万
依托单位：
Southern Illinois University at Carbondale
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-02-15 至 2010-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0349255&HistoricalAwards=false
关键词：
CAREER Development Plan Optical Nuclear

项目摘要

In this project, funded by the Experimental Physical Chemistry Program of the Chemistry Division, Prof. Boyd Goodson of Southern Illinois University at Carbondale will engage in NMR and optical/nuclear double resonance (ONDR) studies of the structure and dynamics of molecular and (bio)macromolecular complexes in liquid-crystalline matrices. The goal is to bring about an improved understanding of host-guest interactions, protein-ligand binding events, and other biological phenomena involving weak but specific interactions. In a second part of this project he will incorporate these research initiatives into the undergraduate physical chemistry curriculum while mentoring undergraduate students in independent research. The research part consists of three related components: 1) the preparation and NMR investigation of inclusion complexes and xenon-protein complexes oriented in various crystalline matrices; 2) the use of laser-polarized xenon to probe interactions with complex-forming molecules and proteins via spin-spin cross-relaxation; and 3) the combination of these two components, whereby the interactions will be amplified by both the optical enhancement of xenon nuclear magnetization and the restoration of dipolar couplings between xenon and host molecules using liquid crystals. The research will be conducted with graduate and undergraduate students. The integration of research into the undergraduate curriculum will emphasize depth over breadth, inquiry-based learning, and exposure to state-of-the-art methods.

在这个项目中，由化学司的实验物理化学计划资助，Carbondale南部伊利诺伊州大学的Boyd Goodson教授将从事NMR和光学/核双共振（ONDR）研究分子和（Bio）液态晶状体矩阵中分子和（Bio）巨型分子复合物的结构和动力学的研究。目的是提高人们对宿主 - 阵线相互作用，蛋白质结合事件以及其他涉及弱但特定相互作用的生物学现象的了解。在该项目的第二部分中，他将将这些研究计划纳入本科生化学课程，同时指导本科生进行独立研究。研究部分由三个相关组成部分组成：1）在各种晶体矩阵中定向的包含复合物和Xenon-蛋白质复合物的制备和NMR研究； 2）使用激光偏振氙气与复合形成分子和蛋白质相互作用，通过自旋旋转交叉浮雕； 3）这两个组件的组合，从而通过使用液体晶体的Xenon核磁化强度的光学增强和Xenon和宿主分子之间的偶极耦合来扩大相互作用。该研究将与研究生和本科生一起进行。在本科课程中的研究集成将强调深度，而不是广度，基于询问的学习以及接触最新方法的深度。