Collaborative Research: Developing Gene-Based Remote Detection

合作研究：开发基于基因的远程检测

• 批准号: 0332918
• 负责人: Joseph Wang
• 金额: $ 15.44万
• 依托单位: New Mexico State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0332918&HistoricalAwards=false
• 关键词: Collaborative; Research; Developing; Gene; Based

Goodwin0332793ABSTRACTThe award provides funds to develop the Phase I Pilot - protocol development stage of a program that the PI's hope will lead to the development and deployment of an in-situ biosensor for remote quantification of aquatic organisms. The biosensor would use electrochemical detection to measure rDNA in the environment without target amplification by the polymerase chair reaction (PCR). Gene-based remote sensing requires the development of easy-to-use, fast, inexpensive, miniaturized analytical devices. Electrochemical detection of DNA hybridization is uniquely qualified to meet size, cost, and power requirements of field systems. This Phase I development will focus on two critical components of biosensor technology development - remote DNA extraction and direct detection of DNA without using PCR amplification. Meeting these two challenges are critical first steps toward developing the next generation of remote sensing technology. Species-specific data generation in conjunction with synoptic measurements of relevant environmental variables could significantly contribute to the understanding of a variety of processes related to plankton dynamics, initiation of algal blooms, and the spread of coral disease. Biosensors to monitor toxic organisms could provide early warnings of the need to close fisheries or beaches.***

GOODWIN0332793ABCRATTER奖项提供了开发I期飞行员的资金 - 计划的协议开发阶段，PI的希望将导致对水生生物远程量化的现场生物传感器的开发和部署。生物传感器将使用电化学检测来测量环境中的rDNA，而无需通过聚合酶椅反应（PCR）靶向扩增。基于基因的遥感需要开发易于使用，快速，廉价，微型分析设备。 DNA杂交的电化学检测具有独特的资格，可以满足现场系统的尺寸，成本和功率要求。 I阶段的开发将集中于生物传感器技术开发的两个关键组成部分 - 远程DNA提取和直接检测DNA，而无需使用PCR扩增。应对这两个挑战是发展下一代遥感技术的关键第一步。物种特异性的数据生成以及相关环境变量的概要测量结果可能会显着有助于理解与浮游动力学相关的多种过程，藻类开花的启动以及珊瑚疾病的传播。监测有毒生物的生物传感器可能会提前警告关闭渔业或海滩的需求。***